Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS07060 and BPHYT_RS07065 are separated by 48 nucleotidesBPHYT_RS07065 and BPHYT_RS07070 are separated by 162 nucleotidesBPHYT_RS07070 and BPHYT_RS07075 are separated by 10 nucleotidesBPHYT_RS07075 and BPHYT_RS07080 are separated by 71 nucleotides BPHYT_RS07060: BPHYT_RS07060 - aldo/keto reductase, at 1,596,947 to 1,597,972 _RS07060 BPHYT_RS07065: BPHYT_RS07065 - 3-ketoacyl-ACP reductase, at 1,598,021 to 1,598,764 _RS07065 BPHYT_RS07070: BPHYT_RS07070 - transcriptional regulator, at 1,598,927 to 1,599,838 _RS07070 BPHYT_RS07075: BPHYT_RS07075 - glyoxalase/bleomycin resistance protein/dioxygenase, at 1,599,849 to 1,600,259 _RS07075 BPHYT_RS07080: BPHYT_RS07080 - glutathione S-transferase, at 1,600,331 to 1,601,341 _RS07080
Group Condition BPHYT_RS07060 BPHYT_RS07065 BPHYT_RS07070 BPHYT_RS07075 BPHYT_RS07080
motility outer cut, LB soft agar motility assay -0.2 +0.1 -0.2 N.D. -2.2
nitrogen source L-Lysine (N) -1.4 -0.3 -0.3 N.D. -0.3
r2a control R2A control -0.5 -0.5 -0.1 N.D. -1.0
nutrient Min media with glucose -0.8 -0.0 -0.1 N.D. -1.0
phage 36hr PHYTO8 MOI 0.1 -0.2 -0.5 -0.2 N.D. -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.2 -0.7 +0.2 N.D. -0.1
carbon source Phenylacetic 5 mM (C) -1.6 -0.2 -0.1 N.D. +0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.2 -0.5 -0.2 N.D. -1.2
carbon source D-Fructose (C) -1.0 -0.4 -0.1 N.D. +0.7
supernatant control Vogels_fungal_media; pH 5.5 -1.9 +0.6 +0.6 N.D. +0.0
phage 36hr PHYTO9 MOI 0.1 +0.9 -0.1 -0.7 N.D. -0.7
phage 24hr PHYTO9 MOI 0.1 -0.8 +0.3 -0.2 N.D. +0.5
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -1.0 +0.4 -0.1 N.D. +0.6
carbon source L-Fucose (C) -0.5 -0.1 -0.5 N.D. +0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.1 +0.7 +0.2 N.D. -1.1
vitamin With vitamins t2 +0.6 +0.3 -0.3 N.D. -0.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.8 -0.3 -0.7 N.D. +0.2
phage 24hr PHYTO6 MOI 10 -0.8 +0.3 -0.3 N.D. +0.9
phage 24hr PHYTO3 MOI 0.1 +0.7 +0.0 -0.8 N.D. +0.4
phage 24hr PHYTO3 MOI 10 -0.3 -0.6 +0.1 N.D. +1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 +0.3 -0.3 N.D. +1.2
phage 36hr PHYTO8 MOI 10 -0.4 +0.5 -0.3 N.D. +0.7
phage 24hr PHYTO6 MOI 1 -0.6 +0.4 +0.3 N.D. +0.5
phage 24hr PHYTO8 MOI 1 +0.6 +0.3 -0.5 N.D. +0.5
phage 36hr PHYTO11 MOI 0.1 +0.4 +0.4 -0.3 N.D. +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.2 +0.5 +0.2 N.D. -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.8 +0.9 +0.4 N.D. -0.3
phage P. phyto PHYTO2 MOI 1 +0.3 +0.7 -0.1 N.D. +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -0.4 -0.2 N.D. +2.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +1.4 +0.5 +0.2 N.D. +0.4
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