Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS06810 and BPHYT_RS06815 are separated by 495 nucleotidesBPHYT_RS06815 and BPHYT_RS06820 are separated by 17 nucleotidesBPHYT_RS06820 and BPHYT_RS06825 are separated by 68 nucleotidesBPHYT_RS06825 and BPHYT_RS06830 are separated by 314 nucleotides BPHYT_RS06810: BPHYT_RS06810 - membrane protein, at 1,539,955 to 1,541,073 _RS06810 BPHYT_RS06815: BPHYT_RS06815 - 16S ribosomal RNA, at 1,541,569 to 1,543,163 _RS06815 BPHYT_RS06820: BPHYT_RS06820 - tRNA-Ile, at 1,543,181 to 1,543,257 _RS06820 BPHYT_RS06825: BPHYT_RS06825 - tRNA-Ala, at 1,543,326 to 1,543,401 _RS06825 BPHYT_RS06830: BPHYT_RS06830 - 23S ribosomal RNA, at 1,543,716 to 1,546,618 _RS06830
Group Condition BPHYT_RS06810 BPHYT_RS06815 BPHYT_RS06820 BPHYT_RS06825 BPHYT_RS06830
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.3 N.D. N.D. N.D. N.D.
phage 36hr PHYTO9 MOI 0.1 -0.3 N.D. N.D. N.D. N.D.
phage 36hr PHYTO1 MOI 10 -0.3 N.D. N.D. N.D. N.D.
carbon source Malonic (C) -0.3 N.D. N.D. N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.3 N.D. N.D. N.D. N.D.
nophagecontrol 36hr Phyto Lib only +0.3 N.D. N.D. N.D. N.D.
stress Bacitracin 0.25 mg/ml +0.3 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 N.D. N.D. N.D. N.D.
phage 36hr PHYTO3 MOI 0.1 +0.3 N.D. N.D. N.D. N.D.
phage P. phyto PHYTO2 MOI 1 +0.3 N.D. N.D. N.D. N.D.
phage P. phyto PHYTO2 MOI 1 +0.3 N.D. N.D. N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.3 N.D. N.D. N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.3 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. N.D. N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. N.D. N.D. N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.4 N.D. N.D. N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.4 N.D. N.D. N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.4 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. N.D. N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.4 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. N.D. N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.4 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 N.D. N.D. N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.6 N.D. N.D. N.D. N.D.
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