Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS06765 and BPHYT_RS06770 are separated by 106 nucleotidesBPHYT_RS06770 and BPHYT_RS06775 are separated by 142 nucleotidesBPHYT_RS06775 and BPHYT_RS06780 are separated by 57 nucleotidesBPHYT_RS06780 and BPHYT_RS06785 are separated by 33 nucleotides BPHYT_RS06765: BPHYT_RS06765 - glutathione S-transferase, at 1,531,032 to 1,531,679 _RS06765 BPHYT_RS06770: BPHYT_RS06770 - aminotransferase, at 1,531,786 to 1,532,958 _RS06770 BPHYT_RS06775: BPHYT_RS06775 - nucleotide-binding protein, at 1,533,101 to 1,533,598 _RS06775 BPHYT_RS06780: BPHYT_RS06780 - hypothetical protein, at 1,533,656 to 1,534,438 _RS06780 BPHYT_RS06785: BPHYT_RS06785 - hypothetical protein, at 1,534,472 to 1,535,626 _RS06785
Group Condition BPHYT_RS06765 BPHYT_RS06770 BPHYT_RS06775 BPHYT_RS06780 BPHYT_RS06785
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.5 -0.1 -0.8 -0.6 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.4 +0.1 -0.6 +0.0 -0.2
carbon source Tween 20 (C) -1.3 +0.1 -0.8 +0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.9 -0.0 -0.9 +0.1 -0.2
motility inner cut, LB soft agar motility assay -0.1 +0.1 -1.2 -0.5 -0.2
phage PHYTO3 MOI 0.1 -0.5 +0.1 -1.0 -0.2 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.0 -0.9 -0.6 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 +0.1 -1.5 +0.2 +0.1
carbon source succinate (C) -0.5 -0.1 -0.4 -0.7 +0.3
phage 24hr PHYTO3 MOI 1 -0.3 +0.3 -2.0 +0.5 +0.1
phage 36hr PHYTO6 MOI 1 -0.9 +0.3 -1.0 +0.3 -0.0
phage 36hr PHYTO8 MOI 10 -0.0 +0.1 -1.6 +0.2 +0.0
phage 36hr PHYTO3 MOI 1 -0.4 +0.2 -1.2 +0.1 +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.2 -0.7 +0.6 -0.2
phage P. phyto PHYTO2 MOI 1 +0.1 -0.1 -1.6 +0.5 +0.2
phage 24hr PHYTO9 MOI 10 +0.5 +0.2 -1.7 +0.3 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 -0.1 -1.6 +0.0 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.3 -0.5 +0.5 -0.3
phage 24hr PHYTO9 MOI 0.1 -0.6 +0.1 +0.5 +0.3 -0.2
phage 24hr PHYTO2 MOI 0.1 -1.1 +0.3 +0.7 +0.3 +0.1
phage 24hr PHYTO10 MOI 1 -0.7 +0.1 +0.6 +0.4 -0.1
phage 24hr PHYTO3 MOI 10 -0.4 -0.0 -0.5 +1.0 +0.3
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.5 +0.1 +1.2 -0.4 +0.5
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.9 +0.2 -0.4 +0.3 +0.0
phage 24hr PHYTO6 MOI 10 -0.4 +0.2 +0.6 +0.5 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.4 +0.2 +0.2 -0.7 +0.1
phage 36hr PHYTO8 MOI 0.1 -0.3 +0.3 +0.3 +0.7 +0.3
phage 36hr PHYTO11 MOI 0.1 -0.1 +0.1 +1.4 +0.4 +0.3
phage 36hr PHYTO7 MOI 1 +0.4 +0.3 +0.9 +0.2 +0.3
phage P. phyto PHYTO2 MOI 1 +0.9 +0.2 +1.3 +0.4 +0.0
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