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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS06765 and BPHYT_RS06770 are separated by 106 nucleotides
BPHYT_RS06770 and BPHYT_RS06775 are separated by 142 nucleotides
BPHYT_RS06775 and BPHYT_RS06780 are separated by 57 nucleotides
BPHYT_RS06780 and BPHYT_RS06785 are separated by 33 nucleotides
BPHYT_RS06765: BPHYT_RS06765 - glutathione S-transferase, at 1,531,032 to 1,531,679
_RS06765
BPHYT_RS06770: BPHYT_RS06770 - aminotransferase, at 1,531,786 to 1,532,958
_RS06770
BPHYT_RS06775: BPHYT_RS06775 - nucleotide-binding protein, at 1,533,101 to 1,533,598
_RS06775
BPHYT_RS06780: BPHYT_RS06780 - hypothetical protein, at 1,533,656 to 1,534,438
_RS06780
BPHYT_RS06785: BPHYT_RS06785 - hypothetical protein, at 1,534,472 to 1,535,626
_RS06785
Group
Condition
BPHYT
_RS06765
BPHYT
_RS06770
BPHYT
_RS06775
BPHYT
_RS06780
BPHYT
_RS06785
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
-0.5
-0.1
-0.8
-0.6
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-1.4
+0.1
-0.6
+0.0
-0.2
carbon source
Tween 20 (C)
-1.3
+0.1
-0.8
+0.2
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.9
-0.0
-0.9
+0.1
-0.2
motility
inner cut, LB soft agar motility assay
-0.1
+0.1
-1.2
-0.5
-0.2
phage
PHYTO3 MOI 0.1
-0.5
+0.1
-1.0
-0.2
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.3
+0.0
-0.9
-0.6
+0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.4
+0.1
-1.5
+0.2
+0.1
carbon source
succinate (C)
-0.5
-0.1
-0.4
-0.7
+0.3
phage
24hr PHYTO3 MOI 1
-0.3
+0.3
-2.0
+0.5
+0.1
phage
36hr PHYTO6 MOI 1
-0.9
+0.3
-1.0
+0.3
-0.0
phage
36hr PHYTO8 MOI 10
-0.0
+0.1
-1.6
+0.2
+0.0
phage
36hr PHYTO3 MOI 1
-0.4
+0.2
-1.2
+0.1
+0.1
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
-0.6
-0.2
-0.7
+0.6
-0.2
phage
P. phyto PHYTO2 MOI 1
+0.1
-0.1
-1.6
+0.5
+0.2
phage
24hr PHYTO9 MOI 10
+0.5
+0.2
-1.7
+0.3
+0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.0
-0.1
-1.6
+0.0
+0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.4
+0.3
-0.5
+0.5
-0.3
phage
24hr PHYTO9 MOI 0.1
-0.6
+0.1
+0.5
+0.3
-0.2
phage
24hr PHYTO2 MOI 0.1
-1.1
+0.3
+0.7
+0.3
+0.1
phage
24hr PHYTO10 MOI 1
-0.7
+0.1
+0.6
+0.4
-0.1
phage
24hr PHYTO3 MOI 10
-0.4
-0.0
-0.5
+1.0
+0.3
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-0.5
+0.1
+1.2
-0.4
+0.5
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
+0.9
+0.2
-0.4
+0.3
+0.0
phage
24hr PHYTO6 MOI 10
-0.4
+0.2
+0.6
+0.5
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.4
+0.2
+0.2
-0.7
+0.1
phage
36hr PHYTO8 MOI 0.1
-0.3
+0.3
+0.3
+0.7
+0.3
phage
36hr PHYTO11 MOI 0.1
-0.1
+0.1
+1.4
+0.4
+0.3
phage
36hr PHYTO7 MOI 1
+0.4
+0.3
+0.9
+0.2
+0.3
phage
P. phyto PHYTO2 MOI 1
+0.9
+0.2
+1.3
+0.4
+0.0
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