Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS06500 and BPHYT_RS06505 are separated by 2 nucleotidesBPHYT_RS06505 and BPHYT_RS06510 are separated by 205 nucleotidesBPHYT_RS06510 and BPHYT_RS06515 are separated by 170 nucleotidesBPHYT_RS06515 and BPHYT_RS06520 are separated by 36 nucleotides BPHYT_RS06500: BPHYT_RS06500 - LOG family protein, at 1,478,186 to 1,478,770 _RS06500 BPHYT_RS06505: BPHYT_RS06505 - TetR family transcriptional regulator, at 1,478,773 to 1,479,498 _RS06505 BPHYT_RS06510: BPHYT_RS06510 - DeoR faimly transcriptional regulator, at 1,479,704 to 1,480,192 _RS06510 BPHYT_RS06515: BPHYT_RS06515 - GDP-mannose-dependent alpha-mannosyltransferase, at 1,480,363 to 1,481,394 _RS06515 BPHYT_RS06520: BPHYT_RS06520 - ser/threonine protein phosphatase, at 1,481,431 to 1,482,372 _RS06520
Group Condition BPHYT_RS06500 BPHYT_RS06505 BPHYT_RS06510 BPHYT_RS06515 BPHYT_RS06520
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.6 -0.5 -0.8 -0.4 -0.1
carbon source Tween 20 (C) +0.6 -2.4 +0.2 -0.5 -0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.4 -0.9 -1.1 -0.6 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.3 -1.1 -0.2 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -2.1 +0.0 +0.3 +0.5 -0.1
carbon source D-Glucose 20 mM (C) +0.5 -0.1 -0.9 -0.4 -0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.3 -1.0 +0.9 -0.1
carbon source D-Glucose (C) -1.3 +0.2 -0.1 +0.5 -0.0
nitrogen source L-Lysine (N) -0.8 -0.8 +0.3 -0.2 +0.7
carbon source Sebacic (C) -1.3 +0.4 +0.6 -0.1 +0.2
carbon source propionate (C) -0.8 -0.3 +0.6 +0.1 +0.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 +0.1 +0.7 -0.3 +0.4
carbon source Protocatechuic Acid (C) -0.9 +0.1 +0.7 +0.3 +0.4
carbon source Malonic (C) -0.9 +0.2 +0.6 +0.2 +0.4
phage P. phyto PHYTO2 MOI 1 -0.3 -0.2 +0.8 +0.6 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.0 +1.4 -0.1 -0.0
phage 36hr PHYTO2 MOI 1 +0.2 +0.2 +1.0 -0.4 -0.4
carbon source Disodium Glutarate (C) -0.9 +0.2 +0.2 +0.4 +0.8
carbon source adipate (C) -0.7 -0.0 +0.7 +0.3 +0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.6 -0.1 +1.4 -0.1 +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.2 -0.5 +1.2 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 +0.0 -0.4 -0.3 +0.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.9 -0.5 +0.6 -0.3 +0.6
carbon source Suberic (C) -0.3 +0.5 +0.8 -0.1 +0.3
carbon source Disodium Glutarate (C) -0.4 +0.3 +0.6 +0.2 +0.8
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.5 -0.3 +0.7 +0.0 +0.6
carbon source Suberic (C) -0.5 +0.6 +0.6 +0.6 +0.5
phage 24hr PHYTO8 MOI 1 +1.0 +0.2 -0.1 +0.3 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.0 +1.5 +0.1 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 -0.2 +1.2 +0.8 -0.1
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