Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS06475 and BPHYT_RS06480 are separated by 92 nucleotidesBPHYT_RS06480 and BPHYT_RS06485 are separated by 26 nucleotidesBPHYT_RS06485 and BPHYT_RS06490 overlap by 8 nucleotidesBPHYT_RS06490 and BPHYT_RS06495 are separated by 164 nucleotides BPHYT_RS06475: BPHYT_RS06475 - membrane protein, at 1,472,987 to 1,473,598 _RS06475 BPHYT_RS06480: BPHYT_RS06480 - ABC transporter permease, at 1,473,691 to 1,476,180 _RS06480 BPHYT_RS06485: BPHYT_RS06485 - globin, at 1,476,207 to 1,476,620 _RS06485 BPHYT_RS06490: BPHYT_RS06490 - membrane protein, at 1,476,613 to 1,477,242 _RS06490 BPHYT_RS06495: BPHYT_RS06495 - glucose-1-dehydrogenase, at 1,477,407 to 1,478,153 _RS06495
Group Condition BPHYT_RS06475 BPHYT_RS06480 BPHYT_RS06485 BPHYT_RS06490 BPHYT_RS06495
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. -0.1 -0.6 -0.3 -0.4
phage 36hr PHYTO2 MOI 1 N.D. +0.0 +0.3 -1.1 -0.5
phage P. phyto corn MOI 1 N.D. +0.1 +0.1 -1.6 +0.1
nitrogen source L-Isoleucine (N) N.D. +0.2 -0.9 +0.0 -0.5
phage P. phyto PHYTO2 MOI 1 N.D. -0.2 +0.8 -1.5 -0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. -0.9 -0.4 +0.0 +0.4
phage 36hr PHYTO6 MOI 10 N.D. -0.2 +0.3 -0.9 +0.1
motility inner cut, LB soft agar motility assay N.D. -0.5 +0.4 -0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.9 -0.2 +0.5
phage 36hr PHYTO10 MOI 10 N.D. -0.2 -0.3 -0.6 +0.5
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.1 -0.5 -0.4 +0.5
carbon source L-Arabinose (C) N.D. -0.4 +0.6 -0.4 -0.2
phage 36hr PHYTO10 MOI 1 N.D. -0.1 -0.1 -0.7 +0.6
phage 36hr PHYTO11 MOI 1 N.D. -0.2 +0.3 -0.6 +0.4
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. +0.3 +0.3 -0.8 +0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -0.3 -0.5 +0.4 +0.4
carbon source L-Proline (C) N.D. -0.1 +0.6 +0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.4 +0.3 -0.9 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.0 +0.9 +0.4 -0.9
phage 36hr PHYTO2 MOI 1 N.D. -0.6 +0.2 +0.4 +0.4
phage 24hr PHYTO2 MOI 0.1 N.D. -0.2 -0.3 +0.6 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. +0.3 -0.2 -0.3 +0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.5 +0.3 +0.7 -0.2
nophagecontrol 24hr Phyto Lib Only N.D. -0.1 +0.4 +0.5 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.1 +0.8 +0.8 +0.0
carbon source Malonic (C) N.D. +0.0 +0.2 +1.0 +0.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. +0.1 +0.4 +0.6 +0.5
nophagecontrol 36hr Phyto Lib only N.D. -0.2 +0.7 +0.5 +0.5
phage 36hr PHYTO8 MOI 10 N.D. +0.1 +0.8 +0.8 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.0 +5.3 -0.3 +0.5
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