Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS06255 and BPHYT_RS06260 are separated by 482 nucleotidesBPHYT_RS06260 and BPHYT_RS06265 are separated by 157 nucleotidesBPHYT_RS06265 and BPHYT_RS06270 are separated by 36 nucleotidesBPHYT_RS06270 and BPHYT_RS06275 are separated by 129 nucleotides BPHYT_RS06255: BPHYT_RS06255 - spore coat protein U, at 1,427,608 to 1,428,132 _RS06255 BPHYT_RS06260: BPHYT_RS06260 - MFS transporter, at 1,428,615 to 1,429,826 _RS06260 BPHYT_RS06265: BPHYT_RS06265 - 3-demethylubiquinone-9 3-methyltransferase, at 1,429,984 to 1,430,457 _RS06265 BPHYT_RS06270: BPHYT_RS06270 - glyoxalase, at 1,430,494 to 1,430,961 _RS06270 BPHYT_RS06275: BPHYT_RS06275 - hydrolase, at 1,431,091 to 1,432,686 _RS06275
Group Condition BPHYT_RS06255 BPHYT_RS06260 BPHYT_RS06265 BPHYT_RS06270 BPHYT_RS06275
phage P. phyto PHYTO2 MOI 1 -1.5 -1.1 -0.4 +0.2 -0.0
phage 24hr PHYTO7 MOI 10 -2.3 +0.1 -0.7 +0.4 -0.1
phage 24hr PHYTO6 MOI 0.1 -1.1 -0.2 -0.8 -0.1 -0.0
phage 24hr PHYTO3 MOI 10 -1.7 -0.6 -0.1 +0.1 +0.0
phage 24hr PHYTO1 MOI 1 -1.5 -0.2 -0.5 -0.0 +0.1
phage 36hr PHYTO9 MOI 0.1 -0.9 +0.0 -0.7 -0.3 -0.2
phage 36hr PHYTO11 MOI 0.1 -1.0 -0.2 -0.5 -0.2 -0.1
phage 36hr PHYTO4 MOI 1 -0.9 -0.5 -0.4 -0.0 -0.3
phage 36hr PHYTO3 MOI 1 -1.6 -0.3 -0.2 +0.2 -0.1
phage 24hr PHYTO MOI 0.1 -1.0 -0.6 -0.4 +0.1 -0.1
phage 36hr PHYTO6 MOI 0.1 -1.0 -0.1 -0.8 +0.0 -0.1
nophagecontrol 36hr Phyto Lib only -1.3 -0.4 -0.4 +0.2 -0.1
phage 36hr PHYTO2 MOI 1 -0.3 -1.0 -0.5 +0.1 -0.1
phage 24hr PHYTO1 MOI 10 -0.1 -1.2 -0.6 +0.1 +0.1
phage 36hr PHYTO8 MOI 0.1 -1.1 +0.1 -0.6 +0.4 -0.1
phage 24hr PHYTO2 MOI 0.1 -0.9 -0.7 +0.3 +0.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -1.0 +0.4 -0.3 +0.3 -0.3
carbon source 2-Deoxy-D-ribonic lithium salt (C) -1.9 -0.0 +0.5 +0.2 +0.3
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -1.0 +0.2 -0.5 +0.4 +0.2
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.9 +0.5 -0.5 +0.1 +0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.5 +0.4 -0.7 +0.3 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.4 -1.0 +0.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 -0.9 -0.1 +0.8 +0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.6 +0.5 +0.4 -0.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.1 +0.4 +1.0 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 +0.5 +0.3 -0.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.6 +0.1 +0.5 +0.6 +0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.0 +1.0 +0.5 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +1.7 -0.4 +0.3 +0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.3 +0.0 +0.7 +0.9 +0.4
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