Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS05810 and BPHYT_RS05815 are separated by 35 nucleotidesBPHYT_RS05815 and BPHYT_RS05820 overlap by 4 nucleotidesBPHYT_RS05820 and BPHYT_RS05825 are separated by 32 nucleotidesBPHYT_RS05825 and BPHYT_RS05830 are separated by 165 nucleotides BPHYT_RS05810: BPHYT_RS05810 - hypothetical protein, at 1,323,255 to 1,324,721 _RS05810 BPHYT_RS05815: BPHYT_RS05815 - hypothetical protein, at 1,324,757 to 1,325,635 _RS05815 BPHYT_RS05820: BPHYT_RS05820 - hypothetical protein, at 1,325,632 to 1,327,530 _RS05820 BPHYT_RS05825: BPHYT_RS05825 - hypothetical protein, at 1,327,563 to 1,327,784 _RS05825 BPHYT_RS05830: BPHYT_RS05830 - hypothetical protein, at 1,327,950 to 1,328,408 _RS05830
Group Condition BPHYT_RS05810 BPHYT_RS05815 BPHYT_RS05820 BPHYT_RS05825 BPHYT_RS05830
phage 24hr PHYTO1 MOI 10 -1.3 +0.0 +0.0 -2.5 -0.4
phage 24hr PHYTO3 MOI 10 -0.3 -0.1 -0.4 -2.7 -0.0
nophagecontrol 24hr Phyto Lib Only -0.4 -0.0 -0.0 -2.6 -0.4
phage P. phyto corn MOI 1 -1.4 -0.2 -0.3 -1.1 -0.3
phage 36hr PHYTO10 MOI 10 -0.1 -0.6 -0.4 -1.6 -0.6
phage 24hr PHYTO2 MOI 0.1 -0.2 -0.0 -0.1 -2.1 -0.7
phage 36hr PHYTO1 MOI 1 -0.8 -0.3 -0.3 -1.2 -0.6
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.0 -0.4 +0.2 -2.9 +0.2
phage 36hr PHYTO11 MOI 0.1 -1.1 -0.2 -0.2 -0.6 -0.5
phage 36hr PHYTO10 MOI 1 -0.8 -0.0 -0.2 -1.1 -0.5
phage 24hr PHYTO6 MOI 10 -0.0 -0.8 +0.1 -1.4 -0.3
phage 36hr PHYTO8 MOI 0.1 +0.2 -0.6 -0.2 -1.9 -0.1
phage 24hr PHYTO3 MOI 10 -0.7 -0.4 -0.1 -0.6 -0.8
phage 24hr PHYTO10 MOI 1 -0.9 -0.1 -0.2 -0.7 -0.6
phage 24hr PHYTO6 MOI 0.1 -0.6 +0.0 -0.2 -1.4 -0.3
carbon source Suberic (C) -1.3 -0.4 -0.3 -0.6 +0.2
carbon source Protocatechuic Acid (C) -0.5 -0.2 -0.5 -1.0 +0.3
phage 36hr PHYTO10 MOI 10 +0.6 -0.1 +0.2 -1.8 -0.5
phage 36hr PHYTO1 MOI 1 -0.8 -0.4 -0.2 +0.5 -0.7
phage P. phyto PHYTO2 MOI 1 +0.5 -0.0 -0.1 -1.4 -0.5
nophagecontrol 24hr Phyto Lib only +0.5 -0.3 +0.1 -1.6 -0.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -1.1 -0.2 +0.2 -0.5 +0.7
phage 36hr PHYTO8 MOI 10 +0.4 -0.4 -0.3 +0.6 -1.1
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.6 -0.5 -0.1 -1.2 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.4 +0.9 -0.1 -0.5 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 +0.9 -0.4 -0.7 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.2 +0.5 -0.2 -0.9 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.6 +0.3 +0.4 +1.5 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.0 +0.4 +0.4 +1.5 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.0 +0.3 +1.0 +2.4 +0.1
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