Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS05790 and BPHYT_RS05795 are separated by 46 nucleotidesBPHYT_RS05795 and BPHYT_RS05800 are separated by 107 nucleotidesBPHYT_RS05800 and BPHYT_RS05805 are separated by 33 nucleotidesBPHYT_RS05805 and BPHYT_RS05810 are separated by 8 nucleotides BPHYT_RS05790: BPHYT_RS05790 - hypothetical protein, at 1,316,362 to 1,317,291 _RS05790 BPHYT_RS05795: BPHYT_RS05795 - hypothetical protein, at 1,317,338 to 1,317,700 _RS05795 BPHYT_RS05800: BPHYT_RS05800 - hypothetical protein, at 1,317,808 to 1,318,089 _RS05800 BPHYT_RS05805: BPHYT_RS05805 - prophage tail length tape measure, at 1,318,123 to 1,323,246 _RS05805 BPHYT_RS05810: BPHYT_RS05810 - hypothetical protein, at 1,323,255 to 1,324,721 _RS05810
Group Condition BPHYT_RS05790 BPHYT_RS05795 BPHYT_RS05800 BPHYT_RS05805 BPHYT_RS05810
phage P. phyto corn MOI 1 -0.7 -0.3 +0.1 -0.3 -1.4
phage 24hr PHYTO9 MOI 0.1 -0.6 +0.0 -0.5 -0.1 -1.2
phage 24hr PHYTO3 MOI 10 -0.4 -0.8 +0.3 -0.1 -0.7
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.4 -0.4 +0.2 +0.1 -1.1
phage 24hr PHYTO3 MOI 10 -1.0 -0.5 +0.5 +0.0 -0.3
carbon source Phenylacetic 5 mM (C) -0.4 -0.2 -1.3 -0.1 +0.6
phage 36hr PHYTO10 MOI 1 -0.4 +0.8 -0.9 +0.0 -0.8
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.2 -0.8 -0.9 +0.1 +0.7
nophagecontrol 36hr Phyto Lib only -1.2 +0.3 -0.2 -0.2 +0.2
phage PHYTO3 MOI 0.1 -0.2 -0.4 +0.9 -0.1 -1.2
phage 24hr PHYTO11 MOI 1 -0.1 +0.9 -0.1 -0.0 -1.2
carbon source propionate (C) -0.4 +0.8 -0.1 +0.1 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.8 +0.1 +0.1 -1.0
phage 24hr PHYTO7 MOI 1 -0.5 +0.7 -0.4 +0.3 -0.2
phage 36hr PHYTO11 MOI 0.1 -0.5 -0.4 +0.8 -0.2 +0.3
carbon source adipate (C) -0.5 +0.8 +0.7 +0.0 -1.1
carbon source succinate (C) +0.0 +0.7 +0.4 -0.1 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.9 +0.6 +0.6 +0.2 -0.2
carbon source Dodecandioic (C) -0.2 +1.0 +0.6 -0.0 -0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +1.1 -0.4 +0.2 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 +0.1 -0.6 +0.2 +1.2
carbon source D-Glucose (C) -0.2 +0.7 +0.9 -0.1 -0.6
phage 36hr PHYTO4 MOI 1 -0.9 +0.8 +0.8 +0.2 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.1 -0.5 +0.2 +1.0
carbon source Suberic (C) -0.3 +1.2 +1.3 +0.1 -1.3
phage 36hr PHYTO2 MOI 1 +0.3 +1.0 +0.6 +0.2 -0.9
carbon source Sebacic (C) +0.0 +0.9 +0.8 -0.0 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.2 +1.0 +0.2 +0.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.5 -0.4 +0.7 -0.0 +0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 +1.0 +0.1 -0.3 +0.5
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