Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS05520 and BPHYT_RS05525 overlap by 17 nucleotidesBPHYT_RS05525 and BPHYT_RS05530 are separated by 91 nucleotidesBPHYT_RS05530 and BPHYT_RS05535 are separated by 211 nucleotidesBPHYT_RS05535 and BPHYT_RS05540 are separated by 708 nucleotides BPHYT_RS05520: BPHYT_RS05520 - transcriptional regulator, at 1,267,307 to 1,268,032 _RS05520 BPHYT_RS05525: BPHYT_RS05525 - sensor histidine kinase, at 1,268,016 to 1,269,335 _RS05525 BPHYT_RS05530: BPHYT_RS05530 - hypothetical protein, at 1,269,427 to 1,269,693 _RS05530 BPHYT_RS05535: BPHYT_RS05535 - diguanylate cyclase, at 1,269,905 to 1,271,596 _RS05535 BPHYT_RS05540: BPHYT_RS05540 - sulfonate ABC transporter permease, at 1,272,305 to 1,274,056 _RS05540
Group Condition BPHYT_RS05520 BPHYT_RS05525 BPHYT_RS05530 BPHYT_RS05535 BPHYT_RS05540
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -1.6 -0.1 -0.6 -0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 -0.6 -0.9 -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.4 -0.9 -2.1 +0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.4 -0.2 -1.0 -0.9 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.6 -0.4 -1.5 -0.2 N.D.
phage 36hr PHYTO2 MOI 0.1 +0.3 -0.4 -1.2 -0.3 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.2 -0.9 -0.8 +0.1 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.7 -1.2 -0.3 -0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.2 -0.1 -0.0 +0.3 N.D.
phage 24hr PHYTO MOI 0.1 -0.4 +0.4 -0.6 -0.3 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.3 -0.6 +0.2 -0.7 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.5 -0.5 -0.3 -0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 +0.4 -0.9 +0.5 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.7 -0.2 -0.5 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 +0.8 -0.4 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 +0.5 +0.3 -0.2 N.D.
phage 36hr PHYTO9 MOI 1 +0.6 +0.3 -0.5 -0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 -0.1 +0.8 +0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.5 +0.7 +0.3 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.7 +0.3 -0.2 +0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 +0.8 -0.0 +0.3 N.D.
phage 36hr PHYTO7 MOI 1 -0.1 -0.5 +0.9 -0.1 N.D.
no phage control No phage control +0.4 +0.6 -0.6 -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 +0.6 +0.8 -0.4 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.7 +0.4 -0.6 +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 -0.1 +1.1 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 +0.3 -0.7 +0.5 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -1.4 -0.8 -0.2 +3.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.5 +0.8 +0.2 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.7 +0.8 +0.2 N.D.
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