Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS05380 and BPHYT_RS05385 are separated by 91 nucleotidesBPHYT_RS05385 and BPHYT_RS05390 are separated by 825 nucleotidesBPHYT_RS05390 and BPHYT_RS05395 are separated by 39 nucleotidesBPHYT_RS05395 and BPHYT_RS05400 are separated by 344 nucleotides BPHYT_RS05380: BPHYT_RS05380 - transcriptional regulator, at 1,237,747 to 1,238,085 _RS05380 BPHYT_RS05385: BPHYT_RS05385 - short-chain dehydrogenase, at 1,238,177 to 1,238,926 _RS05385 BPHYT_RS05390: BPHYT_RS05390 - isochorismatase, at 1,239,752 to 1,240,399 _RS05390 BPHYT_RS05395: BPHYT_RS05395 - hypothetical protein, at 1,240,439 to 1,240,627 _RS05395 BPHYT_RS05400: BPHYT_RS05400 - hypothetical protein, at 1,240,972 to 1,241,418 _RS05400
Group Condition BPHYT_RS05380 BPHYT_RS05385 BPHYT_RS05390 BPHYT_RS05395 BPHYT_RS05400
nitrogen source L-Lysine (N) N.D. -0.4 -1.4 -1.3 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. +0.4 +0.3 -3.2 N.D.
carbon source D-Glucose (C) N.D. -0.1 -1.1 -1.2 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. -0.9 -0.9 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 +0.3 -1.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.3 -1.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.4 -0.4 -0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.2 +0.6 -1.1 N.D.
supernatant control Vogels_fungal_media 0.1X N.D. +0.6 +0.5 -1.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.5 +0.2 -1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.1 +0.7 -1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.3 +1.0 -1.2 N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. +0.4 +0.6 -1.2 N.D.
supernatant control Vogels_fungal_media 0.1X N.D. +0.4 +0.8 -0.6 N.D.
stress Thallium(I) acetate 0.01 mg/ml N.D. +0.3 -0.4 +1.1 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.5 +0.4 +1.1 N.D.
motility inner cut, LB soft agar motility assay N.D. -0.3 +0.8 +0.7 N.D.
stress Paraquat dichloride 0.032 mg/ml N.D. -0.1 +0.2 +1.4 N.D.
phage 36hr PHYTO3 MOI 1 N.D. -0.1 +0.9 +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.5 +0.3 +0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 +1.2 +0.4 N.D.
temperature LB, 23C N.D. +0.3 +0.6 +0.9 N.D.
carbon source Putrescine (C) N.D. +0.2 +0.5 +1.2 N.D.
carbon source D-Galacturonic Acid (C) N.D. +0.2 +0.5 +1.2 N.D.
phage 24hr PHYTO6 MOI 1 N.D. +0.6 +0.4 +1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +2.4 +0.0 -0.3 N.D.
phage 36hr PHYTO2 MOI 1 N.D. +0.8 +0.7 +0.9 N.D.
phage 36hr PHYTO2 MOI 1 N.D. +0.9 +0.9 +0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.5 +0.9 +1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.6 +0.5 +2.1 N.D.
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