Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS05180 and BPHYT_RS05185 overlap by 7 nucleotidesBPHYT_RS05185 and BPHYT_RS05190 are separated by 212 nucleotidesBPHYT_RS05190 and BPHYT_RS05195 are separated by 896 nucleotidesBPHYT_RS05195 and BPHYT_RS05200 are separated by 238 nucleotides BPHYT_RS05180: BPHYT_RS05180 - membrane protein, at 1,192,717 to 1,192,827 _RS05180 BPHYT_RS05185: BPHYT_RS05185 - chloride channel protein, at 1,192,821 to 1,194,245 _RS05185 BPHYT_RS05190: BPHYT_RS05190 - ADP-heptose--LPS heptosyltransferase, at 1,194,458 to 1,195,462 _RS05190 BPHYT_RS05195: BPHYT_RS05195 - hypothetical protein, at 1,196,359 to 1,196,538 _RS05195 BPHYT_RS05200: BPHYT_RS05200 - MFS transporter, at 1,196,777 to 1,198,084 _RS05200
Group Condition BPHYT_RS05180 BPHYT_RS05185 BPHYT_RS05190 BPHYT_RS05195 BPHYT_RS05200
phage 24hr PHYTO9 MOI 0.1 N.D. -0.3 -0.8 -0.9 -0.3
motility inner cut, LB soft agar motility assay N.D. -0.2 -1.4 +0.1 -0.1
motility outer cut, LB soft agar motility assay N.D. -0.2 -1.1 +0.1 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.0 -1.0 +0.2 -0.4
motility outer cut, LB soft agar motility assay N.D. -0.2 -1.3 +0.4 -0.2
nitrogen source L-Lysine (N) N.D. -0.3 +0.3 -0.8 -0.4
phage 24hr PHYTO10 MOI 1 N.D. -0.1 -0.7 +0.6 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.3 -0.8 +0.6 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.2 +0.6 -0.9 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.3 +0.5 -0.8 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.1 -1.0 +1.1 -0.5
phage 36hr PHYTO6 MOI 10 N.D. -0.5 +0.8 -0.0 -0.5
phage 36hr PHYTO8 MOI 10 N.D. -0.4 +1.0 -0.1 -0.5
phage 36hr PHYTO2 MOI 1 N.D. -0.4 +0.6 +0.3 -0.5
pH LB, pH4 N.D. -0.9 +0.7 +0.5 -0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +0.5 +0.5 -0.5 -0.2
pH LB, pH4 N.D. -0.9 +0.6 +0.4 +0.3
phage PHYTO3 MOI 0.1 N.D. -0.3 +1.1 -0.3 +0.1
phage P. phyto corn MOI 1 N.D. -0.3 +1.6 -0.4 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. +0.4 +0.5 -0.4 +0.4
phage 36hr PHYTO10 MOI 10 N.D. -0.4 +1.5 +0.1 -0.1
phage 24hr PHYTO3 MOI 10 N.D. -0.4 +1.8 +0.0 -0.2
phage 24hr PHYTO6 MOI 10 N.D. -0.1 +2.0 -0.3 -0.3
phage 24hr PHYTO3 MOI 10 N.D. +0.0 +1.5 +0.3 -0.4
phage 24hr PHYTO7 MOI 10 N.D. -0.2 +2.1 -0.1 -0.2
stress Bacitracin 0.25 mg/ml N.D. -0.2 +1.6 +0.2 +0.1
carbon source adipate (C) N.D. -0.1 +1.2 +0.3 +0.3
phage P. phyto PHYTO2 MOI 1 N.D. -0.3 +1.7 +0.2 +0.1
phage 24hr PHYTO4 MOI 10 N.D. -0.3 +2.1 -0.0 +0.0
stress Lomefloxacin 0.001 mM N.D. +0.0 +2.0 +0.0 -0.0
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