Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS05160 and BPHYT_RS05165 are separated by 103 nucleotidesBPHYT_RS05165 and BPHYT_RS05170 are separated by 14 nucleotidesBPHYT_RS05170 and BPHYT_RS05175 are separated by 316 nucleotidesBPHYT_RS05175 and BPHYT_RS05180 are separated by 144 nucleotides BPHYT_RS05160: BPHYT_RS05160 - septum site-determining protein MinC, at 1,189,902 to 1,190,756 _RS05160 BPHYT_RS05165: BPHYT_RS05165 - cell division inhibitor MinD, at 1,190,860 to 1,191,675 _RS05165 BPHYT_RS05170: BPHYT_RS05170 - cell division topological specificity factor, at 1,191,690 to 1,191,944 _RS05170 BPHYT_RS05175: BPHYT_RS05175 - hypothetical protein, at 1,192,261 to 1,192,572 _RS05175 BPHYT_RS05180: BPHYT_RS05180 - membrane protein, at 1,192,717 to 1,192,827 _RS05180
Group Condition BPHYT_RS05160 BPHYT_RS05165 BPHYT_RS05170 BPHYT_RS05175 BPHYT_RS05180
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. N.D. -2.5 N.D.
carbon source Suberic (C) N.D. N.D. N.D. -1.8 N.D.
phage 24hr PHYTO9 MOI 0.1 N.D. N.D. N.D. -1.8 N.D.
carbon source Protocatechuic Acid (C) N.D. N.D. N.D. -1.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -1.3 N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. N.D. -1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.1 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.1 N.D.
phage 24hr PHYTO6 MOI 0.1 N.D. N.D. N.D. -1.1 N.D.
phage 24hr PHYTO3 MOI 1 N.D. N.D. N.D. -1.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -1.0 N.D.
carbon source D-Glucose (C) N.D. N.D. N.D. -1.0 N.D.
phage 36hr PHYTO2 MOI 0.1 N.D. N.D. N.D. -1.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.0 N.D.
carbon source Suberic (C) N.D. N.D. N.D. -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. -0.9 N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -0.9 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. N.D. -0.9 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. N.D. -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -0.9 N.D.
carbon source Disodium Glutarate (C) N.D. N.D. N.D. -0.9 N.D.
carbon source Disodium Glutarate (C) N.D. N.D. N.D. -0.9 N.D.
phage P. phyto corn MOI 0.1 N.D. N.D. N.D. -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. N.D. -0.9 N.D.
no phage control No phage control N.D. N.D. N.D. -0.9 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. N.D. -0.9 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. N.D. -0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. N.D. +0.9 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. N.D. +0.9 N.D.
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