Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS04770 and BPHYT_RS04775 are separated by 16 nucleotidesBPHYT_RS04775 and BPHYT_RS04780 are separated by 77 nucleotidesBPHYT_RS04780 and BPHYT_RS04785 are separated by 11 nucleotidesBPHYT_RS04785 and BPHYT_RS04790 are separated by 3 nucleotides BPHYT_RS04770: BPHYT_RS04770 - short-chain dehydrogenase, at 1,101,818 to 1,102,558 _RS04770 BPHYT_RS04775: BPHYT_RS04775 - 2-deoxy-D-ribonate 3-dehydrogenase (from data), at 1,102,575 to 1,103,366 _RS04775 BPHYT_RS04780: BPHYT_RS04780 - cobalamin biosynthesis protein CbiX, at 1,103,444 to 1,103,827 _RS04780 BPHYT_RS04785: BPHYT_RS04785 - permease, at 1,103,839 to 1,104,987 _RS04785 BPHYT_RS04790: BPHYT_RS04790 - permease, at 1,104,991 to 1,106,088 _RS04790
Group Condition BPHYT_RS04770 BPHYT_RS04775 BPHYT_RS04780 BPHYT_RS04785 BPHYT_RS04790
carbon source 2-Deoxy-D-Ribose (C) -0.4 -0.9 N.D. N.D. N.D.
carbon source 2-Deoxy-D-Ribose (C) -0.3 -0.8 N.D. N.D. N.D.
phage 24hr PHYTO6 MOI 10 -0.2 -0.7 N.D. N.D. N.D.
stress Bacitracin 0.25 mg/ml -0.6 -0.3 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.6 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay -0.7 -0.1 N.D. N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.4 -0.4 N.D. N.D. N.D.
phage 36hr PHYTO2 MOI 0.1 -0.5 -0.2 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.8 N.D. N.D. N.D.
supernatant control Vogels_fungal_media 0.1X +0.3 -0.9 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 -0.8 N.D. N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.7 +0.1 N.D. N.D. N.D.
carbon source 2-Deoxy-D-Ribose (C) +0.2 -0.7 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.6 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -0.6 +0.3 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.5 -0.7 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.4 -0.4 N.D. N.D. N.D.
phage 36hr PHYTO11 MOI 0.1 -0.5 +0.5 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 -0.6 N.D. N.D. N.D.
phage 24hr PHYTO2 MOI 0.1 -0.2 +0.7 N.D. N.D. N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.8 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 +0.5 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.2 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.4 N.D. N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) +2.5 -1.7 N.D. N.D. N.D.
vitamin No vitamins t1 +0.3 +0.5 N.D. N.D. N.D.
phage 24hr PHYTO11 MOI 0.1 +0.6 +0.2 N.D. N.D. N.D.
vitamin With vitamins t2 +0.5 +0.4 N.D. N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.6 +0.5 N.D. N.D. N.D.
supernatant control Vogels_fungal_media; pH 5.5 +0.5 +0.7 N.D. N.D. N.D.
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