Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS03995 and BPHYT_RS04000 are separated by 83 nucleotidesBPHYT_RS04000 and BPHYT_RS04005 are separated by 5 nucleotidesBPHYT_RS04005 and BPHYT_RS04010 are separated by 112 nucleotidesBPHYT_RS04010 and BPHYT_RS04015 are separated by 55 nucleotides BPHYT_RS03995: BPHYT_RS03995 - protein tyrosine kinase, at 908,970 to 911,204 _RS03995 BPHYT_RS04000: BPHYT_RS04000 - GDP-mannose 4,6-dehydratase, at 911,288 to 912,406 _RS04000 BPHYT_RS04005: BPHYT_RS04005 - GDP-L-fucose synthase, at 912,412 to 913,371 _RS04005 BPHYT_RS04010: BPHYT_RS04010 - glycosyl transferase, at 913,484 to 914,626 _RS04010 BPHYT_RS04015: BPHYT_RS04015 - glycosyl transferase, at 914,682 to 916,034 _RS04015
Group Condition BPHYT_RS03995 BPHYT_RS04000 BPHYT_RS04005 BPHYT_RS04010 BPHYT_RS04015
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.8 -0.2 -0.6 -3.7 -1.1
phage P. phyto PHYTO2 MOI 1 -0.3 -0.8 -1.9 -0.5 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 -0.7 -0.8 -1.4 -0.1
phage 24hr PHYTO3 MOI 10 -0.6 +0.0 +0.4 -1.7 -1.7
phage 36hr PHYTO6 MOI 10 -0.3 -0.1 -0.1 -2.7 -0.1
phage PHYTO3 MOI 0.1 -0.9 -0.3 -1.0 -1.7 +0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.3 -0.1 -2.3 -1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 -0.5 -0.4 -1.4 +0.6
phage 24hr PHYTO2 MOI 0.1 +0.5 -0.1 -0.3 -3.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 +0.2 -1.2 +0.6 -1.9
phage 36hr PHYTO2 MOI 1 -0.5 -0.3 -0.8 -1.3 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 -0.3 -0.4 -2.3 +1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.6 -0.3 -1.1 -1.1
carbon source Protocatechuic Acid (C) -0.5 -0.1 -2.1 +0.8 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 +0.4 -0.2 -1.8 -0.6
supernatant control Vogels_fungal_media 0.1X +0.1 +0.4 +0.6 -1.0 -1.4
phage 36hr PHYTO1 MOI 1 +0.4 +0.1 +0.4 -3.0 +0.9
phage 36hr PHYTO2 MOI 1 -0.6 -0.2 +0.8 -1.5 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.8 -0.0 -1.1 +2.0
phage 36hr PHYTO10 MOI 10 -0.8 +0.3 +0.2 -1.3 +1.0
phage P. phyto corn MOI 1 -0.6 -0.8 -0.8 +1.2 +0.5
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.7 +0.2 +0.1 -1.3 +1.2
phage 36hr PHYTO6 MOI 0.1 +0.5 +0.2 +1.1 -1.5 +0.6
carbon source Malonic (C) -0.2 +0.1 +0.9 +1.6 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.2 -1.0 +1.8 +1.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.4 -0.5 -0.8 +3.6
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.7 +0.6 +1.2 +1.0 +0.8
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.3 +1.2 +3.5 +0.8 -0.0
carbon source D-Xylose (C) +0.8 +1.0 +1.3 +1.2 +1.0
carbon source Xylitol (C) +0.9 +1.5 +1.6 +1.2 +1.8
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