Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS03925 and BPHYT_RS03930 are separated by 234 nucleotidesBPHYT_RS03930 and BPHYT_RS03935 overlap by 4 nucleotidesBPHYT_RS03935 and BPHYT_RS03940 are separated by 73 nucleotidesBPHYT_RS03940 and BPHYT_RS03945 are separated by 312 nucleotides BPHYT_RS03925: BPHYT_RS03925 - LysR family transcriptional regulator, at 887,441 to 888,334 _RS03925 BPHYT_RS03930: BPHYT_RS03930 - 3-hydroxy-3-methylglutaryl-CoA lyase, at 888,569 to 889,546 _RS03930 BPHYT_RS03935: BPHYT_RS03935 - CoA transferase, at 889,543 to 890,781 _RS03935 BPHYT_RS03940: BPHYT_RS03940 - MFS transporter, at 890,855 to 892,339 _RS03940 BPHYT_RS03945: BPHYT_RS03945 - hypothetical protein, at 892,652 to 893,005 _RS03945
Group Condition BPHYT_RS03925 BPHYT_RS03930 BPHYT_RS03935 BPHYT_RS03940 BPHYT_RS03945
nophagecontrol 36hr Phyto Lib only -0.8 -0.5 -0.0 -0.3 -1.4
phage 36hr PHYTO11 MOI 0.1 -0.6 -0.4 +0.2 -0.0 -1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 -0.2 +0.4 -0.2 -1.6
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.9 +0.2 +0.1 +0.1 -1.2
phage 36hr PHYTO8 MOI 10 +0.5 -0.0 +0.2 -0.0 -2.1
carbon source Tween 20 (C) -0.9 +0.1 +0.2 +0.3 -0.9
phage 36hr PHYTO2 MOI 1 +0.4 +0.6 +0.4 -0.3 -2.2
phage 24hr PHYTO9 MOI 0.1 -0.4 -0.4 -1.0 -0.1 +0.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.4 +0.2 +0.8 -0.7
phage 36hr PHYTO9 MOI 1 +0.6 +0.3 +0.3 -0.1 -1.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -1.3 -0.4 -0.2 -0.1 +1.4
phage 24hr PHYTO7 MOI 10 -0.5 -0.5 -0.3 -0.1 +0.8
phage 24hr PHYTO6 MOI 0.1 +0.6 -0.8 -0.3 -0.4 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +1.1 -0.2 -0.3 +0.1 -1.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.3 +0.8 -0.4 +0.2 -0.7
phage 36hr PHYTO7 MOI 1 +0.5 +0.4 +0.0 -0.4 -0.8
nitrogen source NAG (N) -1.0 -0.3 +0.4 +0.1 +0.7
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.4 -0.1 +0.3 +0.3 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 +0.3 +0.2 -0.5 +0.8
carbon source Malonic (C) +0.7 +0.4 -0.5 -0.3 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.6 +0.2 +0.7 -0.3 +0.8
phage 24hr PHYTO2 MOI 0.1 +0.7 +0.6 +0.2 -0.3 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +0.4 -0.6 -0.1 +0.7
nitrogen source Putrescine (N) -0.2 +0.6 +0.2 -0.3 +0.9
supernatant control Vogels_fungal_media; pH 5.5 -0.0 -0.4 +0.7 +0.6 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.1 -0.2 +0.5 +0.2 +1.4
motility outer cut, LB soft agar motility assay +0.9 +0.5 -0.0 +0.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +1.7 +0.4 +0.0 -0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 +0.7 +0.4 +0.1 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.2 +0.5 +0.3 +0.4 +1.4
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