Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS03715 and BPHYT_RS03720 are separated by 70 nucleotidesBPHYT_RS03720 and BPHYT_RS03725 are separated by 516 nucleotidesBPHYT_RS03725 and BPHYT_RS03730 are separated by 6 nucleotidesBPHYT_RS03730 and BPHYT_RS03735 are separated by 198 nucleotides BPHYT_RS03715: BPHYT_RS03715 - 4-hydroxybenzoyl-CoA thioesterase, at 843,456 to 843,935 _RS03715 BPHYT_RS03720: BPHYT_RS03720 - malonic semialdehyde reductase, at 844,006 to 844,752 _RS03720 BPHYT_RS03725: BPHYT_RS03725 - serine hydroxymethyltransferase, at 845,269 to 846,516 _RS03725 BPHYT_RS03730: BPHYT_RS03730 - transcriptional repressor NrdR, at 846,523 to 847,005 _RS03730 BPHYT_RS03735: BPHYT_RS03735 - pilus assembly protein, at 847,204 to 847,770 _RS03735
Group Condition BPHYT_RS03715 BPHYT_RS03720 BPHYT_RS03725 BPHYT_RS03730 BPHYT_RS03735
vitamin With vitamins t1 +0.1 -0.2 -3.8 -2.7 +0.1
lb LB +0.1 -0.2 -4.6 -1.5 -0.0
nitrogen source Ammonium chloride (N) -0.2 -0.0 -4.1 -1.0 -0.5
nitrogen source L-Lysine (N) +0.2 -0.3 -4.1 -0.6 -0.4
nitrogen source L-Serine (N) -0.2 +0.1 -4.1 -0.4 -0.5
vitamin No vitamins t2 +0.1 -0.2 -3.7 -1.0 -0.3
carbon source L-Fucose (C) -0.2 +0.0 -4.3 -0.7 -0.0
vitamin No vitamins t1 +0.1 -0.4 -3.8 -0.9 -0.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 +0.1 -3.5 -1.0 +0.1
nitrogen source L-Phenylalanine (N) -0.3 +0.1 -4.0 -0.6 -0.2
nitrogen source L-Alanine (N) +0.0 +0.2 -4.0 -1.2 +0.0
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.6 +0.2 -0.7 -4.9 +0.0
vitamin With vitamins t2 +0.1 +0.1 -3.6 -1.3 +0.0
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.1 +1.0 -3.0 -2.7 +0.1
nitrogen source L-Asparagine (N) -0.2 +0.4 -4.1 -0.5 -0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.1 +0.5 -3.8 -0.8 -0.1
nitrogen source L-Citrulline (N) +0.1 +0.4 -4.0 -0.4 -0.3
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.1 -0.2 -1.7 -2.8 +0.3
nitrogen source Uridine (N) -0.1 +0.4 -4.0 -0.6 +0.1
motility outer cut, LB soft agar motility assay -0.1 +0.2 -4.2 -0.3 +0.3
carbon source Trisodium citrate (C) +0.1 +0.5 -3.5 -1.3 +0.1
carbon source Xylitol (C) +0.3 +0.3 -3.4 -0.9 +0.3
nitrogen source L-Glutamic (N) +0.0 +0.6 -4.0 +0.4 -0.1
carbon source L-Histidine (C) +0.1 -0.0 -4.2 +0.9 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.2 +0.5 -3.8 -0.6 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 +0.8 -3.5 -0.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.5 +0.3 -3.4 +0.0 -0.2
carbon source L-Glutamine (C) -0.2 -0.0 -3.3 +1.7 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 +1.0 -3.4 +0.3 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.2 +0.6 -3.3 +2.4 -1.1
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