Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS03660 and BPHYT_RS03665 are separated by 233 nucleotidesBPHYT_RS03665 and BPHYT_RS03670 are separated by 490 nucleotidesBPHYT_RS03670 and BPHYT_RS03675 are separated by 239 nucleotidesBPHYT_RS03675 and BPHYT_RS03680 are separated by 1204 nucleotides BPHYT_RS03660: BPHYT_RS03660 - mechanosensitive ion channel protein MscS, at 832,108 to 832,920 _RS03660 BPHYT_RS03665: BPHYT_RS03665 - hypothetical protein, at 833,154 to 833,519 _RS03665 BPHYT_RS03670: BPHYT_RS03670 - peptidase U34, at 834,010 to 835,467 _RS03670 BPHYT_RS03675: BPHYT_RS03675 - porin, at 835,707 to 836,924 _RS03675 BPHYT_RS03680: BPHYT_RS03680 - tRNA-Lys, at 838,129 to 838,204 _RS03680
Group Condition BPHYT_RS03660 BPHYT_RS03665 BPHYT_RS03670 BPHYT_RS03675 BPHYT_RS03680
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.7 -0.3 -0.3 N.D.
nitrogen source L-Lysine (N) -0.7 -0.0 -0.2 -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.6 -0.2 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.0 +0.0 -0.1 +0.5 N.D.
nitrogen source D-Alanine (N) -0.6 -0.4 +0.1 +0.1 N.D.
nitrogen source Carnitine Hydrochloride (N) -0.4 -0.5 +0.1 +0.2 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.9 +0.6 -0.1 -0.3 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X +0.5 -1.0 -0.0 -0.0 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -1.1 +0.8 +0.0 -0.2 N.D.
motility inner cut, LB soft agar motility assay -0.6 +0.5 -0.1 +0.0 N.D.
phage 24hr PHYTO2 MOI 0.1 -0.6 +0.5 -0.1 +0.1 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 -0.4 -0.2 -0.3 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.4 +0.3 -0.3 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 +0.6 -0.2 +0.2 N.D.
phage 36hr PHYTO10 MOI 10 +0.5 +0.3 -0.2 -0.2 N.D.
nitrogen source Putrescine (N) -0.4 +0.4 +0.2 +0.3 N.D.
phage 24hr PHYTO4 MOI 10 +0.7 -0.3 -0.1 +0.1 N.D.
carbon source Protocatechuic Acid (C) +0.5 +0.3 -0.2 -0.2 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.5 -0.3 +0.2 +0.2 N.D.
nophagecontrol 36hr Phyto Lib only +0.4 +0.5 -0.0 -0.2 N.D.
lb_dap plate control LB_DAP agar plate, outgrowth in LB +0.6 +0.3 +0.2 -0.2 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.2 +1.2 -0.1 +0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.2 +0.2 +0.4 N.D.
phage P. phyto corn MOI 0.1 +0.4 +0.7 +0.1 -0.1 N.D.
phage 24hr PHYTO7 MOI 10 -0.2 +1.2 -0.1 +0.2 N.D.
phage 24hr PHYTO8 MOI 1 +0.6 +0.8 -0.1 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 +0.6 -0.1 +0.0 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.1 +1.2 +0.1 +0.1 N.D.
supernatant control Vogels_fungal_media; pH 5.5 +1.0 +0.4 +0.0 +0.1 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.4 +1.2 +0.4 -0.0 N.D.
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