Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS03645 and BPHYT_RS03650 are separated by 45 nucleotidesBPHYT_RS03650 and BPHYT_RS03655 are separated by 206 nucleotidesBPHYT_RS03655 and BPHYT_RS03660 are separated by 131 nucleotidesBPHYT_RS03660 and BPHYT_RS03665 are separated by 233 nucleotides BPHYT_RS03645: BPHYT_RS03645 - tRNA dimethylallyltransferase, at 828,042 to 828,989 _RS03645 BPHYT_RS03650: BPHYT_RS03650 - DNA mismatch repair protein, at 829,035 to 831,098 _RS03650 BPHYT_RS03655: BPHYT_RS03655 - membrane protein, at 831,305 to 831,976 _RS03655 BPHYT_RS03660: BPHYT_RS03660 - mechanosensitive ion channel protein MscS, at 832,108 to 832,920 _RS03660 BPHYT_RS03665: BPHYT_RS03665 - hypothetical protein, at 833,154 to 833,519 _RS03665
Group Condition BPHYT_RS03645 BPHYT_RS03650 BPHYT_RS03655 BPHYT_RS03660 BPHYT_RS03665
phage 24hr PHYTO1 MOI 10 -4.0 +0.2 -2.4 -0.1 -0.3
phage 24hr PHYTO10 MOI 10 -3.7 +0.2 -2.2 +0.1 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -4.6 -0.4 -0.9 +0.1 -0.1
nitrogen source Ammonium chloride (N) -5.2 +0.2 -0.5 -0.3 +0.1
nitrogen source NAG (N) -4.9 +0.0 +0.2 -0.8 -0.2
phage 24hr PHYTO2 MOI 1 -2.9 +0.2 -2.5 -0.1 -0.3
phage 24hr PHYTO10 MOI 10 -3.8 +0.6 -2.3 -0.0 -0.2
motility outer cut, LB soft agar motility assay -2.1 -0.3 -2.8 -0.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -3.1 -0.2 -1.6 -0.4 -0.1
phage 36hr PHYTO10 MOI 10 -3.4 +0.5 -2.0 -0.2 -0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -3.0 +1.3 -3.5 -0.1 +0.2
nitrogen source D-Alanine (N) -2.0 +0.1 -2.1 -0.6 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -4.4 -0.2 -0.7 +0.3 +0.2
carbon source L-Lactate (C) -5.0 -0.1 -0.0 +0.1 +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -3.3 +0.2 -2.3 +0.5 +0.2
carbon source Sebacic (C) -5.0 -0.0 +0.8 +0.1 -0.3
carbon source D-Glucose (C) -4.6 -0.1 -0.1 +0.2 +0.3
phage 36hr PHYTO1 MOI 10 -2.9 +0.2 -2.3 +0.7 +0.0
motility inner cut, LB soft agar motility assay -1.0 +0.4 -3.5 -0.6 +0.5
carbon source 2-Deoxy-D-ribonic lithium salt (C) -3.7 +0.5 -1.0 +0.4 -0.2
carbon source D-Glucose 20 mM (C) -5.0 -0.1 +1.0 -0.1 +0.2
carbon source Protocatechuic Acid (C) -4.0 -0.2 +0.8 -0.1 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X -4.8 +0.1 +1.0 +0.2 -0.1
supernatant control Vogels_fungal_media; pH 5.5 -4.8 -0.4 +0.4 +1.0 +0.4
vitamin With vitamins t2 -4.2 +0.2 +1.2 -0.4 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -4.2 +0.1 +1.0 -0.3 +0.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -3.8 +0.1 +0.9 +0.5 +0.2
stress Bacitracin 0.25 mg/ml +1.5 +0.5 -3.4 +0.3 -0.0
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -3.5 +0.2 +0.8 +0.4 +1.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -3.4 +3.1 -1.1 +0.1 +0.5
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