Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS03385 and BPHYT_RS03390 are separated by 70 nucleotidesBPHYT_RS03390 and BPHYT_RS03395 are separated by 218 nucleotidesBPHYT_RS03395 and BPHYT_RS03400 are separated by 783 nucleotidesBPHYT_RS03400 and BPHYT_RS03405 are separated by 148 nucleotides BPHYT_RS03385: BPHYT_RS03385 - DeoR family transcriptional regulator, at 761,732 to 762,472 _RS03385 BPHYT_RS03390: BPHYT_RS03390 - glyoxalase, at 762,543 to 762,926 _RS03390 BPHYT_RS03395: BPHYT_RS03395 - DNA methyltransferase, at 763,145 to 764,446 _RS03395 BPHYT_RS03400: BPHYT_RS03400 - ATP-dependent DNA helicase RecG, at 765,230 to 767,680 _RS03400 BPHYT_RS03405: BPHYT_RS03405 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 767,829 to 768,887 _RS03405
Group Condition BPHYT_RS03385 BPHYT_RS03390 BPHYT_RS03395 BPHYT_RS03400 BPHYT_RS03405
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.7 -0.1 -0.3 -3.0 +0.2
pH LB, pH7 -0.4 -0.2 -0.1 -3.5 +0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.1 +0.5 -0.2 -2.9 -1.4
supernatant control Vogels_fungal_media 0.1X +0.1 -0.4 +0.1 -3.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.3 -0.2 -0.3 -2.3 -0.5
motility outer cut, LB soft agar motility assay +0.2 +0.2 -0.5 -3.2 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.0 -0.1 -0.5 -2.1 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 +0.5 -0.1 -1.9 -1.5
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.4 -0.4 +0.2 -3.2 -0.3
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.0 -0.3 +0.3 -3.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.2 +0.5 -0.3 -1.6 -1.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.3 -0.1 -0.2 -1.5 -1.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.4 +0.6 +0.0 -2.4 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.1 -0.2 -1.6 -1.7
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.4 +0.8 -0.2 -2.2 -1.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.1 -0.3 -1.4 -1.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.7 -0.4 -0.1 -2.1 -0.7
motility outer cut, LB soft agar motility assay +0.3 +0.4 -0.2 -3.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.1 +0.0 -1.8 -1.3
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.5 -0.6 +0.4 -2.0 -0.7
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.0 +0.2 +0.5 -1.7 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 -0.3 +0.0 -2.2 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 +0.3 +0.2 -2.0 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 +0.1 +0.3 -2.0 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 +0.3 +0.1 -1.4 -1.5
motility inner cut, LB soft agar motility assay +0.3 -0.2 -0.1 -3.1 +1.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.9 +0.7 +0.1 -2.9 -0.0
carbon source D-Glucosamine Hydrochloride (C) -0.0 +0.2 -0.0 -2.3 +1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.2 +0.5 +0.5 -2.6 +0.0
phage 24hr PHYTO8 MOI 1 +0.3 -0.3 +0.2 +2.0 +0.6
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