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Cofit
Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS03220 and BPHYT_RS03225 are separated by 128 nucleotides
BPHYT_RS03225 and BPHYT_RS03230 are separated by 189 nucleotides
BPHYT_RS03230 and BPHYT_RS03235 are separated by 118 nucleotides
BPHYT_RS03235 and BPHYT_RS03240 are separated by 140 nucleotides
BPHYT_RS03220: BPHYT_RS03220 - Acyl-CoA dehydrogenase (EC 1.3.8.7)
(from data)
, at 721,553 to 723,340
_RS03220
BPHYT_RS03225: BPHYT_RS03225 - 3-hydroxyacyl-CoA dehydrogenase, at 723,469 to 725,904
_RS03225
BPHYT_RS03230: BPHYT_RS03230 - acetyl-CoA acetyltransferase, at 726,094 to 727,293
_RS03230
BPHYT_RS03235: BPHYT_RS03235 - enoyl-CoA hydratase, at 727,412 to 728,182
_RS03235
BPHYT_RS03240: BPHYT_RS03240 - formate dehydrogenase accessory protein FdhD, at 728,323 to 729,162
_RS03240
Group
Condition
BPHYT
_RS03220
BPHYT
_RS03225
BPHYT
_RS03230
BPHYT
_RS03235
BPHYT
_RS03240
carbon source
Tween 20 (C)
-2.7
-1.4
-0.8
+0.2
-0.2
carbon source
m-Inositol (C)
-0.5
-0.3
-0.5
-1.1
-0.3
motility
outer cut, LB soft agar motility assay
-0.1
-0.1
-0.5
-0.8
-1.1
phage
36hr PHYTO4 MOI 1
+0.3
+0.2
+0.3
-2.6
-0.6
carbon source
Trisodium citrate (C)
-1.1
-0.2
-0.4
-0.5
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.0
-0.3
-0.0
-1.6
-0.4
phage
24hr PHYTO4 MOI 10
+0.1
+0.3
-0.0
-1.9
-0.8
motility
outer cut, LB soft agar motility assay
-0.0
+0.0
-0.5
-0.3
-1.4
carbon source
Sebacic (C)
+0.4
-0.1
+0.0
-1.2
-1.0
pH
LB, pH4
+0.0
+0.3
-0.2
-0.4
-1.4
phage
36hr PHYTO2 MOI 1
+0.1
+0.3
-0.4
-1.3
-0.5
in planta
Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
+0.9
-0.0
-0.4
-0.7
-1.4
nophagecontrol
24hr Phyto Lib Only
+0.1
+0.1
+0.1
-0.4
-1.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.1
+0.4
+0.1
-0.3
-1.4
phage
P. phyto PHYTO2 MOI 0.1
+0.1
-0.2
-0.3
+0.2
-1.3
phage
24hr PHYTO7 MOI 1
-0.0
-0.0
-0.4
+0.9
-1.6
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.1
+0.2
-0.7
-0.8
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-0.2
+0.4
+0.5
+0.2
-2.0
vitamin
No vitamins t1
-0.2
+0.0
-0.4
-1.1
+0.8
in planta
Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1
+0.8
+0.3
+0.0
-0.4
-1.3
nophagecontrol
36hr Phyto Lib only
+0.2
+0.3
-0.3
+0.3
-1.1
phage
24hr PHYTO3 MOI 10
+0.5
+0.1
-0.4
+0.3
-1.1
phage
36hr PHYTO6 MOI 10
+0.3
+0.4
+0.3
-0.9
-0.6
phage
24hr PHYTO8 MOI 1
-0.3
+0.2
-0.6
+0.6
-0.4
carbon source
2-Deoxy-D-ribonic lithium salt (C)
-0.1
-0.1
+0.7
-1.1
+0.1
phage
24hr PHYTO10 MOI 10
+0.3
+0.2
+0.4
-0.5
-0.7
phage
24hr PHYTO2 MOI 1
+0.2
+0.3
+0.5
-1.2
+0.0
stress
Bacitracin 0.25 mg/ml
+0.3
+0.5
+0.3
-0.7
-0.3
stress
Paraquat dichloride 0.064 mg/ml
+1.4
+0.2
+0.2
-0.1
-0.7
phage
36hr PHYTO2 MOI 1
+0.3
+0.6
+0.4
+0.6
-0.7
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