Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS03220 and BPHYT_RS03225 are separated by 128 nucleotidesBPHYT_RS03225 and BPHYT_RS03230 are separated by 189 nucleotidesBPHYT_RS03230 and BPHYT_RS03235 are separated by 118 nucleotidesBPHYT_RS03235 and BPHYT_RS03240 are separated by 140 nucleotides BPHYT_RS03220: BPHYT_RS03220 - Acyl-CoA dehydrogenase (EC 1.3.8.7) (from data), at 721,553 to 723,340 _RS03220 BPHYT_RS03225: BPHYT_RS03225 - 3-hydroxyacyl-CoA dehydrogenase, at 723,469 to 725,904 _RS03225 BPHYT_RS03230: BPHYT_RS03230 - acetyl-CoA acetyltransferase, at 726,094 to 727,293 _RS03230 BPHYT_RS03235: BPHYT_RS03235 - enoyl-CoA hydratase, at 727,412 to 728,182 _RS03235 BPHYT_RS03240: BPHYT_RS03240 - formate dehydrogenase accessory protein FdhD, at 728,323 to 729,162 _RS03240
Group Condition BPHYT_RS03220 BPHYT_RS03225 BPHYT_RS03230 BPHYT_RS03235 BPHYT_RS03240
carbon source Tween 20 (C) -2.7 -1.4 -0.8 +0.2 -0.2
carbon source m-Inositol (C) -0.5 -0.3 -0.5 -1.1 -0.3
motility outer cut, LB soft agar motility assay -0.1 -0.1 -0.5 -0.8 -1.1
phage 36hr PHYTO4 MOI 1 +0.3 +0.2 +0.3 -2.6 -0.6
carbon source Trisodium citrate (C) -1.1 -0.2 -0.4 -0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 -0.3 -0.0 -1.6 -0.4
phage 24hr PHYTO4 MOI 10 +0.1 +0.3 -0.0 -1.9 -0.8
motility outer cut, LB soft agar motility assay -0.0 +0.0 -0.5 -0.3 -1.4
carbon source Sebacic (C) +0.4 -0.1 +0.0 -1.2 -1.0
pH LB, pH4 +0.0 +0.3 -0.2 -0.4 -1.4
phage 36hr PHYTO2 MOI 1 +0.1 +0.3 -0.4 -1.3 -0.5
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.9 -0.0 -0.4 -0.7 -1.4
nophagecontrol 24hr Phyto Lib Only +0.1 +0.1 +0.1 -0.4 -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.4 +0.1 -0.3 -1.4
phage P. phyto PHYTO2 MOI 0.1 +0.1 -0.2 -0.3 +0.2 -1.3
phage 24hr PHYTO7 MOI 1 -0.0 -0.0 -0.4 +0.9 -1.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.1 +0.2 -0.7 -0.8
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.2 +0.4 +0.5 +0.2 -2.0
vitamin No vitamins t1 -0.2 +0.0 -0.4 -1.1 +0.8
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.8 +0.3 +0.0 -0.4 -1.3
nophagecontrol 36hr Phyto Lib only +0.2 +0.3 -0.3 +0.3 -1.1
phage 24hr PHYTO3 MOI 10 +0.5 +0.1 -0.4 +0.3 -1.1
phage 36hr PHYTO6 MOI 10 +0.3 +0.4 +0.3 -0.9 -0.6
phage 24hr PHYTO8 MOI 1 -0.3 +0.2 -0.6 +0.6 -0.4
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.1 -0.1 +0.7 -1.1 +0.1
phage 24hr PHYTO10 MOI 10 +0.3 +0.2 +0.4 -0.5 -0.7
phage 24hr PHYTO2 MOI 1 +0.2 +0.3 +0.5 -1.2 +0.0
stress Bacitracin 0.25 mg/ml +0.3 +0.5 +0.3 -0.7 -0.3
stress Paraquat dichloride 0.064 mg/ml +1.4 +0.2 +0.2 -0.1 -0.7
phage 36hr PHYTO2 MOI 1 +0.3 +0.6 +0.4 +0.6 -0.7
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