Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS02965 and BPHYT_RS02970 are separated by 37 nucleotidesBPHYT_RS02970 and BPHYT_RS02975 are separated by 386 nucleotidesBPHYT_RS02975 and BPHYT_RS02980 are separated by 135 nucleotidesBPHYT_RS02980 and BPHYT_RS02985 are separated by 296 nucleotides BPHYT_RS02965: BPHYT_RS02965 - membrane protein, at 658,575 to 659,660 _RS02965 BPHYT_RS02970: BPHYT_RS02970 - excinuclease ABC subunit A, at 659,698 to 662,571 _RS02970 BPHYT_RS02975: BPHYT_RS02975 - membrane protein, at 662,958 to 664,115 _RS02975 BPHYT_RS02980: BPHYT_RS02980 - single-stranded DNA-binding protein, at 664,251 to 664,790 _RS02980 BPHYT_RS02985: BPHYT_RS02985 - MaoC family dehydratase, at 665,087 to 665,587 _RS02985
Group Condition BPHYT_RS02965 BPHYT_RS02970 BPHYT_RS02975 BPHYT_RS02980 BPHYT_RS02985
carbon source 2-Deoxy-D-ribonic lithium salt (C) -1.3 -0.1 -2.3 N.D. -5.2
tn7 efficiency Outgrowth after Tn7 transformation -5.5 +0.3 -1.2 N.D. +0.0
motility outer cut, LB soft agar motility assay -4.2 +0.7 -2.7 N.D. -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.4 +0.0 -2.8 N.D. -1.1
stress Zinc 0.5 mM -3.7 -0.3 -0.4 N.D. -0.4
motility outer cut, LB soft agar motility assay -3.9 +0.8 -1.4 N.D. -0.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -3.7 +0.3 -1.3 N.D. -0.1
stress Chloride 200 mM -3.4 +0.0 -0.6 N.D. -0.8
motility inner cut, LB soft agar motility assay -3.6 +0.6 -2.4 N.D. +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.5 -0.2 -2.9 N.D. +0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -2.2 -0.0 -3.3 N.D. +1.3
lb_dap plate control LB_DAP agar plate, outgrowth in LB -2.4 +0.2 -1.6 N.D. -0.4
nitrogen source L-Lysine (N) -3.9 +0.0 +0.4 N.D. -0.6
motility inner cut, LB soft agar motility assay -3.0 +0.6 -1.7 N.D. +0.2
motility outer cut, LB soft agar motility assay -3.2 +0.7 -1.1 N.D. -0.3
carbon source L-Arginine (C) -4.0 +0.2 -0.2 N.D. +0.2
carbon source propionate (C) -3.1 +0.3 -1.0 N.D. +0.1
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -2.8 +0.2 -1.2 N.D. +0.3
tn7 efficiency Outgrowth after Tn7 transformation -3.5 +0.1 -1.0 N.D. +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 +0.1 -2.7 N.D. +0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -1.5 -0.2 -1.6 N.D. +1.1
stress Benzethonium chloride 0.0016 mM -3.6 +0.9 +1.0 N.D. -0.5
phage 36hr PHYTO3 MOI 1 -0.3 +0.0 +1.2 N.D. -3.2
pH LB, pH4 +1.1 +0.5 -2.5 N.D. +0.5
temperature LB, 37C +1.5 +0.9 -2.1 N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.3 +0.6 -1.2 N.D. -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.1 +0.8 -1.6 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.6 +0.9 -1.3 N.D. -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +2.7 +1.2 -0.2 N.D. -0.3
phage 24hr PHYTO4 MOI 10 +1.1 +0.6 +2.9 N.D. +0.3
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