Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS02445 and BPHYT_RS02450 are separated by 311 nucleotidesBPHYT_RS02450 and BPHYT_RS02455 are separated by 314 nucleotidesBPHYT_RS02455 and BPHYT_RS02460 are separated by 41 nucleotidesBPHYT_RS02460 and BPHYT_RS02465 overlap by 4 nucleotides BPHYT_RS02445: BPHYT_RS02445 - lipoyl synthase, at 554,935 to 555,942 _RS02445 BPHYT_RS02450: BPHYT_RS02450 - hypothetical protein, at 556,254 to 556,700 _RS02450 BPHYT_RS02455: BPHYT_RS02455 - taurine ABC transporter substrate-binding protein, at 557,015 to 557,998 _RS02455 BPHYT_RS02460: BPHYT_RS02460 - nitrate ABC transporter ATP-binding protein, at 558,040 to 558,837 _RS02460 BPHYT_RS02465: BPHYT_RS02465 - taurine ABC transporter permease, at 558,834 to 559,703 _RS02465
Group Condition BPHYT_RS02445 BPHYT_RS02450 BPHYT_RS02455 BPHYT_RS02460 BPHYT_RS02465
phage 24hr PHYTO3 MOI 10 N.D. N.D. -0.6 +0.0 -0.4
phage 24hr PHYTO1 MOI 1 N.D. N.D. -0.3 -0.3 -0.3
phage 24hr PHYTO9 MOI 10 N.D. N.D. -0.1 -0.4 -0.4
phage 24hr PHYTO2 MOI 1 N.D. N.D. -0.6 -0.4 +0.1
carbon source Suberic (C) N.D. N.D. -0.2 -0.2 -0.4
phage 24hr PHYTO6 MOI 10 N.D. N.D. -0.3 +0.2 -0.6
nitrogen source L-Lysine (N) N.D. N.D. -0.3 -0.5 +0.1
phage P. phyto PHYTO2 MOI 1 N.D. N.D. +0.2 -0.5 -0.3
phage 36hr PHYTO MOI 0.1 N.D. N.D. -0.6 +0.2 -0.0
carbon source L-Glutamine (C) N.D. N.D. -0.1 +0.2 -0.5
phage 24hr PHYTO10 MOI 1 N.D. N.D. +0.3 -0.3 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.1 +0.3 -0.4
phage 24hr PHYTO8 MOI 1 N.D. N.D. -0.5 +0.1 +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.4 +0.1 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. -0.1 -0.3 +0.4
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. -0.1 -0.3 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.2 +0.3 -0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. +0.3 -0.3 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.4 -0.2 +0.2
nitrogen source L-Serine (N) N.D. N.D. -0.2 -0.0 +0.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. +0.4 +0.3 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. +0.5 +0.2 -0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. +0.7 -0.0 -0.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. N.D. -0.2 +0.2 +0.7
tn7 efficiency Outgrowth after Tn7 transformation N.D. N.D. +0.5 +0.2 +0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. +0.1 +0.3 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.6 +0.2 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. +0.2 +0.2 +0.4
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. +0.2 +0.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.1 +0.3 +0.9
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