Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS02350 and BPHYT_RS02355 are separated by 561 nucleotidesBPHYT_RS02355 and BPHYT_RS02360 are separated by 155 nucleotidesBPHYT_RS02360 and BPHYT_RS02365 are separated by 71 nucleotidesBPHYT_RS02365 and BPHYT_RS02370 are separated by 53 nucleotides BPHYT_RS02350: BPHYT_RS02350 - hypothetical protein, at 538,778 to 539,599 _RS02350 BPHYT_RS02355: BPHYT_RS02355 - ferritin, at 540,161 to 541,033 _RS02355 BPHYT_RS02360: BPHYT_RS02360 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 541,189 to 541,671 _RS02360 BPHYT_RS02365: BPHYT_RS02365 - hypothetical protein, at 541,743 to 541,982 _RS02365 BPHYT_RS02370: BPHYT_RS02370 - type III pantothenate kinase, at 542,036 to 542,848 _RS02370
Group Condition BPHYT_RS02350 BPHYT_RS02355 BPHYT_RS02360 BPHYT_RS02365 BPHYT_RS02370
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. N.D. -2.9 N.D.
phage 24hr PHYTO3 MOI 10 N.D. N.D. N.D. -2.7 N.D.
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. N.D. -2.4 N.D.
phage P. phyto PHYTO2 MOI 1 N.D. N.D. N.D. -2.4 N.D.
phage 24hr PHYTO7 MOI 1 N.D. N.D. N.D. -2.3 N.D.
carbon source D-Glucose (C) N.D. N.D. N.D. -2.1 N.D.
phage 24hr PHYTO8 MOI 1 N.D. N.D. N.D. -2.0 N.D.
carbon source Tween 20 (C) N.D. N.D. N.D. -1.9 N.D.
phage 36hr PHYTO10 MOI 10 N.D. N.D. N.D. -1.8 N.D.
phage 36hr PHYTO1 MOI 1 N.D. N.D. N.D. -1.8 N.D.
supernatant control Vogels_fungal_media 0.1X N.D. N.D. N.D. -1.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. N.D. -1.7 N.D.
phage 24hr PHYTO3 MOI 10 N.D. N.D. N.D. -1.7 N.D.
phage 36hr PHYTO2 MOI 0.1 N.D. N.D. N.D. -1.7 N.D.
vitamin With vitamins t2 N.D. N.D. N.D. -1.7 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. N.D. N.D. -1.7 N.D.
carbon source Malonic (C) N.D. N.D. N.D. -1.6 N.D.
carbon source L-Arabinose (C) N.D. N.D. N.D. -1.6 N.D.
phage 36hr PHYTO4 MOI 1 N.D. N.D. N.D. -1.5 N.D.
phage 36hr PHYTO9 MOI 0.1 N.D. N.D. N.D. -1.5 N.D.
nophagecontrol 24hr Phyto Lib only N.D. N.D. N.D. -1.4 N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. N.D. N.D. -1.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -1.3 N.D.
nophagecontrol 36hr Phyto Lib only N.D. N.D. N.D. -1.3 N.D.
phage 36hr PHYTO11 MOI 1 N.D. N.D. N.D. -1.3 N.D.
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. N.D. -1.3 N.D.
phage 24hr PHYTO3 MOI 0.1 N.D. N.D. N.D. -1.2 N.D.
vitamin No vitamins t1 N.D. N.D. N.D. -1.2 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. N.D. -1.2 N.D.
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. N.D. +1.5 N.D.
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