Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS02345 and BPHYT_RS02350 are separated by 97 nucleotidesBPHYT_RS02350 and BPHYT_RS02355 are separated by 561 nucleotidesBPHYT_RS02355 and BPHYT_RS02360 are separated by 155 nucleotidesBPHYT_RS02360 and BPHYT_RS02365 are separated by 71 nucleotides BPHYT_RS02345: BPHYT_RS02345 - enoyl-CoA hydratase, at 537,793 to 538,680 _RS02345 BPHYT_RS02350: BPHYT_RS02350 - hypothetical protein, at 538,778 to 539,599 _RS02350 BPHYT_RS02355: BPHYT_RS02355 - ferritin, at 540,161 to 541,033 _RS02355 BPHYT_RS02360: BPHYT_RS02360 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 541,189 to 541,671 _RS02360 BPHYT_RS02365: BPHYT_RS02365 - hypothetical protein, at 541,743 to 541,982 _RS02365
Group Condition BPHYT_RS02345 BPHYT_RS02350 BPHYT_RS02355 BPHYT_RS02360 BPHYT_RS02365
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.2 N.D. N.D. N.D. -2.9
phage 24hr PHYTO11 MOI 0.1 +0.1 N.D. N.D. N.D. -2.4
phage P. phyto PHYTO2 MOI 1 +0.1 N.D. N.D. N.D. -2.4
phage 24hr PHYTO3 MOI 10 +0.4 N.D. N.D. N.D. -2.7
phage 24hr PHYTO7 MOI 1 +0.1 N.D. N.D. N.D. -2.3
vitamin With vitamins t2 -0.3 N.D. N.D. N.D. -1.7
carbon source Malonic (C) -0.3 N.D. N.D. N.D. -1.6
phage 36hr PHYTO10 MOI 10 -0.0 N.D. N.D. N.D. -1.8
phage 24hr PHYTO8 MOI 1 +0.1 N.D. N.D. N.D. -2.0
phage 36hr PHYTO1 MOI 1 -0.0 N.D. N.D. N.D. -1.8
supernatant control Vogels_fungal_media 0.1X +0.1 N.D. N.D. N.D. -1.8
carbon source D-Glucose (C) +0.5 N.D. N.D. N.D. -2.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 N.D. N.D. N.D. -1.7
phage 36hr PHYTO4 MOI 1 +0.1 N.D. N.D. N.D. -1.5
carbon source Tween 20 (C) +0.5 N.D. N.D. N.D. -1.9
phage 24hr PHYTO3 MOI 10 +0.3 N.D. N.D. N.D. -1.7
phage 36hr PHYTO2 MOI 0.1 +0.4 N.D. N.D. N.D. -1.7
carbon source L-Arabinose (C) +0.3 N.D. N.D. N.D. -1.6
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.5 N.D. N.D. N.D. -1.7
nophagecontrol 24hr Phyto Lib only +0.2 N.D. N.D. N.D. -1.4
phage 36hr PHYTO9 MOI 0.1 +0.3 N.D. N.D. N.D. -1.5
carbon source 2-Deoxy-D-Ribose (C) +0.2 N.D. N.D. N.D. -1.4
nophagecontrol 36hr Phyto Lib only +0.3 N.D. N.D. N.D. -1.3
phage 24hr PHYTO4 MOI 0.1 +0.3 N.D. N.D. N.D. -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 N.D. N.D. N.D. -1.3
phage 36hr PHYTO11 MOI 1 +0.3 N.D. N.D. N.D. -1.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.8 N.D. N.D. N.D. +0.8
supernatant control Vogels_fungal_media; pH 5.5 -0.2 N.D. N.D. N.D. +1.5
carbon source adipate (C) +0.7 N.D. N.D. N.D. +1.1
carbon source Suberic (C) +0.7 N.D. N.D. N.D. +1.2
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