Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS02335 and BPHYT_RS02340 are separated by 253 nucleotidesBPHYT_RS02340 and BPHYT_RS02345 are separated by 49 nucleotidesBPHYT_RS02345 and BPHYT_RS02350 are separated by 97 nucleotidesBPHYT_RS02350 and BPHYT_RS02355 are separated by 561 nucleotides BPHYT_RS02335: BPHYT_RS02335 - fumarylacetoacetate hydrolase, at 535,271 to 536,254 _RS02335 BPHYT_RS02340: BPHYT_RS02340 - branched-chain amino acid ABC transporter substrate-binding protein, at 536,508 to 537,743 _RS02340 BPHYT_RS02345: BPHYT_RS02345 - enoyl-CoA hydratase, at 537,793 to 538,680 _RS02345 BPHYT_RS02350: BPHYT_RS02350 - hypothetical protein, at 538,778 to 539,599 _RS02350 BPHYT_RS02355: BPHYT_RS02355 - ferritin, at 540,161 to 541,033 _RS02355
Group Condition BPHYT_RS02335 BPHYT_RS02340 BPHYT_RS02345 BPHYT_RS02350 BPHYT_RS02355
carbon source D-Lactate (C) -0.4 -0.4 -0.2 N.D. N.D.
carbon source D-Glucose (C) -0.4 -0.2 -0.3 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.0 +0.0 -0.8 N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.4 +0.2 -0.5 N.D. N.D.
supernatant control Vogels_fungal_media 0.1X +0.2 -0.2 -0.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.5 +0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.7 -0.1 -0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.3 +0.3 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.5 +0.2 N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.1 +0.1 +0.6 N.D. N.D.
phage 24hr PHYTO3 MOI 10 -0.2 +0.4 +0.3 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 -0.2 +0.6 N.D. N.D.
motility outer cut, LB soft agar motility assay +0.3 -0.1 +0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.3 +0.3 N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.1 -0.0 +0.7 N.D. N.D.
carbon source adipate (C) +0.1 +0.0 +0.7 N.D. N.D.
carbon source m-Inositol (C) +0.2 +0.3 +0.4 N.D. N.D.
carbon source L-Fucose (C) +0.2 +0.2 +0.5 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.3 +0.2 +0.5 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.2 +0.0 +0.6 N.D. N.D.
temperature LB, 37C +0.1 +0.3 +0.5 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.4 +0.3 +0.3 N.D. N.D.
stress Nickel (II) chloride 1 mM +0.2 +0.2 +0.5 N.D. N.D.
carbon source D-Glucose (C) +0.4 +0.1 +0.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.5 +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.1 +0.7 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.7 +0.1 +0.3 N.D. N.D.
carbon source Suberic (C) +0.2 +0.3 +0.7 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.1 +0.2 -0.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +0.4 +0.6 N.D. N.D.
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