Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS02230 and BPHYT_RS02235 overlap by 8 nucleotidesBPHYT_RS02235 and BPHYT_RS02240 are separated by 94 nucleotidesBPHYT_RS02240 and BPHYT_RS02245 are separated by 85 nucleotidesBPHYT_RS02245 and BPHYT_RS02250 are separated by 187 nucleotides BPHYT_RS02230: BPHYT_RS02230 - acetyl-CoA acetyltransferase, at 512,101 to 513,294 _RS02230 BPHYT_RS02235: BPHYT_RS02235 - beta carbonic anhydrase, at 513,287 to 514,063 _RS02235 BPHYT_RS02240: BPHYT_RS02240 - isocitrate dehydrogenase kinase/phosphatase, at 514,158 to 515,996 _RS02240 BPHYT_RS02245: BPHYT_RS02245 - MerR family transcriptional regulator, at 516,082 to 516,486 _RS02245 BPHYT_RS02250: BPHYT_RS02250 - Zn-dependent hydrolase, at 516,674 to 517,747 _RS02250
Group Condition BPHYT_RS02230 BPHYT_RS02235 BPHYT_RS02240 BPHYT_RS02245 BPHYT_RS02250
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 -0.1 -0.3 -1.9 +0.2
carbon source Suberic (C) +0.0 -0.1 -0.3 -1.5 -0.4
phage 36hr PHYTO1 MOI 10 -0.0 -0.7 +0.1 -1.5 +0.2
carbon source Tween 20 (C) -0.1 +0.5 -1.9 -0.1 -0.3
phage 36hr PHYTO8 MOI 10 +0.3 -0.0 +0.0 -2.1 -0.0
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.3 -0.7 +0.0 -1.3 +0.5
carbon source Suberic (C) -0.2 +0.4 -0.1 -1.8 +0.2
phage 24hr PHYTO11 MOI 0.1 -0.0 +0.4 -0.2 -1.4 -0.1
phage 24hr PHYTO6 MOI 1 +0.8 +0.3 -0.0 -2.3 -0.2
carbon source Azelaic (C) -0.1 +0.5 -0.6 -0.9 -0.1
phage 24hr PHYTO4 MOI 10 +0.2 +0.5 -0.1 -1.6 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.8 +0.2 +0.2 -0.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 +0.0 +0.1 -1.1 +0.5
phage 36hr PHYTO11 MOI 0.1 +0.4 -0.2 +0.1 -1.3 +0.1
phage 36hr PHYTO10 MOI 10 +0.3 -0.1 +0.0 -1.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 +0.6 -0.2 -0.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.4 -0.0 -1.3 -0.2
phage 36hr PHYTO3 MOI 1 +0.6 +0.3 -0.1 -1.4 -0.2
phage 24hr PHYTO1 MOI 1 +0.4 -0.1 +0.0 -1.4 +0.3
phage 36hr PHYTO11 MOI 1 +0.7 -0.2 -0.2 -0.8 +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.3 +0.4 -0.0 -1.1 +0.7
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 -0.9 +0.3 +0.8 -0.6
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.2 +1.2 -0.1 -0.4 -0.5
carbon source Xylitol (C) +0.4 +0.2 -0.1 -1.0 +0.6
phage P. phyto corn MOI 0.1 +0.4 +0.3 +0.1 -1.0 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 -0.2 -0.1 +1.6 -0.2
phage 24hr PHYTO6 MOI 0.1 +0.7 +0.5 +0.1 -0.7 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.1 +0.5 +0.3 +1.1 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +1.3 -0.2 +0.7 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.4 +0.5 +0.4 +1.1 +0.3
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