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Protein
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Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS02075 and BPHYT_RS02080 are separated by 205 nucleotides
BPHYT_RS02080 and BPHYT_RS02085 are separated by 82 nucleotides
BPHYT_RS02085 and BPHYT_RS02090 are separated by 224 nucleotides
BPHYT_RS02090 and BPHYT_RS02095 are separated by 283 nucleotides
BPHYT_RS02075: BPHYT_RS02075 - porin, at 475,485 to 476,570
_RS02075
BPHYT_RS02080: BPHYT_RS02080 - LysR family transcriptional regulator, at 476,776 to 477,714
_RS02080
BPHYT_RS02085: BPHYT_RS02085 - methylmalonyl-CoA mutase, at 477,797 to 481,693
_RS02085
BPHYT_RS02090: BPHYT_RS02090 - 2-nitropropane dioxygenase, at 481,918 to 482,877
_RS02090
BPHYT_RS02095: BPHYT_RS02095 - alpha/beta hydrolase, at 483,161 to 484,051
_RS02095
Group
Condition
BPHYT
_RS02075
BPHYT
_RS02080
BPHYT
_RS02085
BPHYT
_RS02090
BPHYT
_RS02095
r2a control
R2A control
-0.1
-0.5
-0.2
-0.4
-0.3
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
-0.2
-0.0
-0.3
-0.2
-0.4
phage
36hr PHYTO8 MOI 10
-0.1
-0.5
+0.1
-0.2
-0.5
phage
36hr PHYTO8 MOI 10
+0.1
-0.0
-0.4
+0.0
-0.6
phage
P. phyto PHYTO2 MOI 1
-0.1
-0.6
-0.1
+0.3
-0.3
phage
24hr PHYTO9 MOI 10
-0.0
+0.2
-0.1
-0.3
-0.4
phage
36hr PHYTO10 MOI 10
+0.0
+0.3
-0.3
+0.1
-0.7
nophagecontrol
36hr Phyto Lib only
-0.0
-0.6
+0.2
+0.1
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
-0.1
+0.1
-0.1
-0.6
phage
24hr PHYTO3 MOI 10
+0.2
+0.1
-0.2
+0.1
-0.5
lb_dap plate control
LB_DAP agar plate, outgrowth in LB
-0.0
-0.6
+0.1
-0.1
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
+0.2
-0.4
+0.1
-0.4
+0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days
+0.4
-0.3
-0.0
-0.4
+0.4
carbon source
Tween 20 (C)
+0.0
+0.4
+0.1
+0.1
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.1
-0.4
+0.2
+0.3
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
-0.5
+0.2
+0.2
+0.4
motility
outer cut, LB soft agar motility assay
+0.3
+0.3
+0.1
-0.5
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
+0.1
+0.3
+0.2
+0.3
-0.5
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
+0.2
-0.4
-0.1
+0.1
+0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
+0.5
+0.3
-0.1
-0.2
supernatant control
Vogels_fungal_media 0.1X
+0.1
-0.1
+0.3
+0.3
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.0
-0.1
+0.5
+0.3
+0.2
nutrient
L-Histidine
+0.1
+0.5
+0.1
+0.3
+0.2
in planta
Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
+0.3
+0.6
+0.2
-0.2
+0.2
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
+0.4
+0.2
+0.0
+0.2
+0.4
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
+0.0
+0.1
+0.4
+0.1
+0.6
supernatant control
Vogels_fungal_media 0.1X
+0.2
+0.4
+0.1
+0.2
+0.4
supernatant control
Vogels_fungal_media; pH 5.5
+0.2
+0.7
+0.0
+0.2
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X
+0.1
+0.1
+0.2
+0.3
+0.6
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.2
+0.4
+0.2
+0.4
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