Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS02075 and BPHYT_RS02080 are separated by 205 nucleotidesBPHYT_RS02080 and BPHYT_RS02085 are separated by 82 nucleotidesBPHYT_RS02085 and BPHYT_RS02090 are separated by 224 nucleotidesBPHYT_RS02090 and BPHYT_RS02095 are separated by 283 nucleotides BPHYT_RS02075: BPHYT_RS02075 - porin, at 475,485 to 476,570 _RS02075 BPHYT_RS02080: BPHYT_RS02080 - LysR family transcriptional regulator, at 476,776 to 477,714 _RS02080 BPHYT_RS02085: BPHYT_RS02085 - methylmalonyl-CoA mutase, at 477,797 to 481,693 _RS02085 BPHYT_RS02090: BPHYT_RS02090 - 2-nitropropane dioxygenase, at 481,918 to 482,877 _RS02090 BPHYT_RS02095: BPHYT_RS02095 - alpha/beta hydrolase, at 483,161 to 484,051 _RS02095
Group Condition BPHYT_RS02075 BPHYT_RS02080 BPHYT_RS02085 BPHYT_RS02090 BPHYT_RS02095
r2a control R2A control -0.1 -0.5 -0.2 -0.4 -0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.2 -0.0 -0.3 -0.2 -0.4
phage 36hr PHYTO8 MOI 10 -0.1 -0.5 +0.1 -0.2 -0.5
phage 36hr PHYTO8 MOI 10 +0.1 -0.0 -0.4 +0.0 -0.6
phage P. phyto PHYTO2 MOI 1 -0.1 -0.6 -0.1 +0.3 -0.3
phage 24hr PHYTO9 MOI 10 -0.0 +0.2 -0.1 -0.3 -0.4
phage 36hr PHYTO10 MOI 10 +0.0 +0.3 -0.3 +0.1 -0.7
nophagecontrol 36hr Phyto Lib only -0.0 -0.6 +0.2 +0.1 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.1 +0.1 -0.1 -0.6
phage 24hr PHYTO3 MOI 10 +0.2 +0.1 -0.2 +0.1 -0.5
lb_dap plate control LB_DAP agar plate, outgrowth in LB -0.0 -0.6 +0.1 -0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.2 -0.4 +0.1 -0.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.4 -0.3 -0.0 -0.4 +0.4
carbon source Tween 20 (C) +0.0 +0.4 +0.1 +0.1 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 -0.4 +0.2 +0.3 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 -0.5 +0.2 +0.2 +0.4
motility outer cut, LB soft agar motility assay +0.3 +0.3 +0.1 -0.5 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.1 +0.3 +0.2 +0.3 -0.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.2 -0.4 -0.1 +0.1 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.5 +0.3 -0.1 -0.2
supernatant control Vogels_fungal_media 0.1X +0.1 -0.1 +0.3 +0.3 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 -0.1 +0.5 +0.3 +0.2
nutrient L-Histidine +0.1 +0.5 +0.1 +0.3 +0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.3 +0.6 +0.2 -0.2 +0.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.4 +0.2 +0.0 +0.2 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.0 +0.1 +0.4 +0.1 +0.6
supernatant control Vogels_fungal_media 0.1X +0.2 +0.4 +0.1 +0.2 +0.4
supernatant control Vogels_fungal_media; pH 5.5 +0.2 +0.7 +0.0 +0.2 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.1 +0.1 +0.2 +0.3 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.2 +0.4 +0.2 +0.4
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