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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS01860 and BPHYT_RS01865 are separated by 235 nucleotides
BPHYT_RS01865 and BPHYT_RS01870 are separated by 108 nucleotides
BPHYT_RS01870 and BPHYT_RS01875 are separated by 284 nucleotides
BPHYT_RS01875 and BPHYT_RS01880 are separated by 252 nucleotides
BPHYT_RS01860: BPHYT_RS01860 - high potential iron-sulfur protein, at 422,750 to 423,061
_RS01860
BPHYT_RS01865: BPHYT_RS01865 - major facilitator transporter, at 423,297 to 424,589
_RS01865
BPHYT_RS01870: BPHYT_RS01870 - 6-aminohexanoate-cyclic-dimer hydrolase, at 424,698 to 426,218
_RS01870
BPHYT_RS01875: BPHYT_RS01875 - carboxymethylenebutenolidase, at 426,503 to 427,201
_RS01875
BPHYT_RS01880: BPHYT_RS01880 - nitronate monooxygenase, at 427,454 to 428,554
_RS01880
Group
Condition
BPHYT
_RS01860
BPHYT
_RS01865
BPHYT
_RS01870
BPHYT
_RS01875
BPHYT
_RS01880
carbon source
Suberic (C)
-0.5
+0.0
-0.5
-0.2
-0.5
phage
PHYTO3 MOI 0.1
-0.4
-0.2
+0.0
-0.7
-0.1
phage
24hr PHYTO2 MOI 1
-0.5
+0.0
-0.2
-0.5
-0.0
phage
24hr PHYTO6 MOI 0.1
-0.5
+0.0
-0.1
-0.6
-0.1
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-0.8
-0.3
-0.2
-0.0
+0.1
carbon source
Sebacic (C)
-0.4
+0.2
-0.3
-0.5
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.3
-0.1
+0.1
-0.3
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.0
+0.0
+0.3
-0.4
+0.0
carbon source
Protocatechuic Acid (C)
-0.4
-0.2
+0.2
-0.4
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
-0.5
+0.1
-0.6
+0.1
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.2
-0.3
-0.2
-0.4
-0.1
phage
24hr PHYTO2 MOI 0.1
-0.4
+0.0
+0.3
-0.5
-0.1
nophagecontrol
36hr Phyto Lib Only
-0.3
+0.2
+0.2
-0.6
-0.1
lb_dap plate control and outgrow on gent
LB_DAP agar plate, outgrowth in LB_gent
-0.5
-0.1
+0.4
-0.3
-0.1
phage
24hr PHYTO9 MOI 10
+0.2
+0.2
+0.0
-0.8
+0.1
carbon source
2-Deoxy-D-ribonic lithium salt (C)
-0.9
-0.0
+0.3
+0.2
+0.2
phage
36hr PHYTO6 MOI 10
-0.6
+0.4
+0.1
-0.0
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.3
+0.0
-0.1
-0.7
+0.3
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
+0.1
-0.2
+0.3
-0.5
+0.2
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
+0.4
+0.1
-0.2
-0.5
+0.2
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.0
-0.1
-0.3
-0.2
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
+0.1
+0.5
-0.6
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
+0.1
+0.5
-0.6
+0.1
motility
inner cut, LB soft agar motility assay
-0.3
+0.1
-0.3
+0.7
+0.1
lb_dap plate control and outgrow on gent
LB_DAP agar plate, outgrowth in LB_gent
+0.3
-0.2
+0.5
-0.3
+0.1
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
+0.3
-0.2
+0.4
-0.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
-0.1
+0.6
-0.2
-0.1
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days
+0.4
-0.2
+0.2
-0.1
+0.4
supernatant control
Vogels_fungal_media 0.1X
+0.3
+0.1
-0.1
+0.5
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.2
-0.0
+0.6
+0.5
+0.4
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