Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS01860 and BPHYT_RS01865 are separated by 235 nucleotidesBPHYT_RS01865 and BPHYT_RS01870 are separated by 108 nucleotidesBPHYT_RS01870 and BPHYT_RS01875 are separated by 284 nucleotidesBPHYT_RS01875 and BPHYT_RS01880 are separated by 252 nucleotides BPHYT_RS01860: BPHYT_RS01860 - high potential iron-sulfur protein, at 422,750 to 423,061 _RS01860 BPHYT_RS01865: BPHYT_RS01865 - major facilitator transporter, at 423,297 to 424,589 _RS01865 BPHYT_RS01870: BPHYT_RS01870 - 6-aminohexanoate-cyclic-dimer hydrolase, at 424,698 to 426,218 _RS01870 BPHYT_RS01875: BPHYT_RS01875 - carboxymethylenebutenolidase, at 426,503 to 427,201 _RS01875 BPHYT_RS01880: BPHYT_RS01880 - nitronate monooxygenase, at 427,454 to 428,554 _RS01880
Group Condition BPHYT_RS01860 BPHYT_RS01865 BPHYT_RS01870 BPHYT_RS01875 BPHYT_RS01880
carbon source Suberic (C) -0.5 +0.0 -0.5 -0.2 -0.5
phage PHYTO3 MOI 0.1 -0.4 -0.2 +0.0 -0.7 -0.1
phage 24hr PHYTO2 MOI 1 -0.5 +0.0 -0.2 -0.5 -0.0
phage 24hr PHYTO6 MOI 0.1 -0.5 +0.0 -0.1 -0.6 -0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.8 -0.3 -0.2 -0.0 +0.1
carbon source Sebacic (C) -0.4 +0.2 -0.3 -0.5 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 -0.1 +0.1 -0.3 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.0 +0.0 +0.3 -0.4 +0.0
carbon source Protocatechuic Acid (C) -0.4 -0.2 +0.2 -0.4 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.5 +0.1 -0.6 +0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 -0.3 -0.2 -0.4 -0.1
phage 24hr PHYTO2 MOI 0.1 -0.4 +0.0 +0.3 -0.5 -0.1
nophagecontrol 36hr Phyto Lib Only -0.3 +0.2 +0.2 -0.6 -0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.5 -0.1 +0.4 -0.3 -0.1
phage 24hr PHYTO9 MOI 10 +0.2 +0.2 +0.0 -0.8 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.9 -0.0 +0.3 +0.2 +0.2
phage 36hr PHYTO6 MOI 10 -0.6 +0.4 +0.1 -0.0 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +0.0 -0.1 -0.7 +0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.1 -0.2 +0.3 -0.5 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.4 +0.1 -0.2 -0.5 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 -0.1 -0.3 -0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 +0.1 +0.5 -0.6 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.1 +0.5 -0.6 +0.1
motility inner cut, LB soft agar motility assay -0.3 +0.1 -0.3 +0.7 +0.1
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.3 -0.2 +0.5 -0.3 +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.2 +0.4 -0.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.1 +0.6 -0.2 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.4 -0.2 +0.2 -0.1 +0.4
supernatant control Vogels_fungal_media 0.1X +0.3 +0.1 -0.1 +0.5 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 -0.0 +0.6 +0.5 +0.4
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