Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS01625 and BPHYT_RS01630 are separated by 143 nucleotidesBPHYT_RS01630 and BPHYT_RS01635 are separated by 9 nucleotidesBPHYT_RS01635 and BPHYT_RS01640 are separated by 478 nucleotidesBPHYT_RS01640 and BPHYT_RS01645 are separated by 677 nucleotides BPHYT_RS01625: BPHYT_RS01625 - chemotaxis protein CheY, at 369,437 to 369,979 _RS01625 BPHYT_RS01630: BPHYT_RS01630 - ATP-dependent protease ATPase subunit HslU, at 370,123 to 371,469 _RS01630 BPHYT_RS01635: BPHYT_RS01635 - ATP-dependent protease subunit HslV, at 371,479 to 372,015 _RS01635 BPHYT_RS01640: BPHYT_RS01640 - molecular chaperone DnaK, at 372,494 to 372,913 _RS01640 BPHYT_RS01645: BPHYT_RS01645 - cobalamin biosynthesis protein CobW, at 373,591 to 374,679 _RS01645
Group Condition BPHYT_RS01625 BPHYT_RS01630 BPHYT_RS01635 BPHYT_RS01640 BPHYT_RS01645
pH LB, pH4 -5.2 -0.7 N.D. N.D. -0.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -4.7 -0.9 N.D. N.D. -0.4
carbon source Phenylacetic 5 mM (C) -4.4 -1.3 N.D. N.D. -0.2
motility inner cut, LB soft agar motility assay -4.4 -1.7 N.D. N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -3.9 -1.7 N.D. N.D. -0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -4.9 -1.1 N.D. N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.9 -3.0 N.D. N.D. +0.1
motility outer cut, LB soft agar motility assay -4.5 -1.0 N.D. N.D. +0.3
motility outer cut, LB soft agar motility assay -3.9 -1.8 N.D. N.D. +0.5
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -4.7 -0.1 N.D. N.D. -0.3
pH LB, pH4 -4.5 -0.6 N.D. N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -4.2 -0.5 N.D. N.D. -0.1
carbon source L-Arginine (C) -4.1 -0.7 N.D. N.D. +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -4.2 -0.2 N.D. N.D. -0.3
phage PHYTO3 MOI 0.1 -2.4 -2.6 N.D. N.D. +0.3
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -4.8 -0.1 N.D. N.D. +0.2
stress Cephalothin 2 mg/ml -4.4 -0.1 N.D. N.D. -0.1
phage 36hr PHYTO9 MOI 0.1 -2.7 -1.0 N.D. N.D. -0.8
stress Paraquat dichloride 0.064 mg/ml +1.0 -5.2 N.D. N.D. -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.1 -1.8 N.D. N.D. +0.8
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -3.5 -0.5 N.D. N.D. -0.0
lb LB -3.5 -0.4 N.D. N.D. -0.2
motility outer cut, LB soft agar motility assay -3.6 -0.4 N.D. N.D. +0.1
temperature LB, 30C -3.1 -0.6 N.D. N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.4 -0.4 N.D. N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.0 -0.9 N.D. N.D. +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -4.0 +0.3 N.D. N.D. +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -3.5 +0.9 N.D. N.D. +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -3.3 +0.7 N.D. N.D. +0.5
phage 36hr PHYTO8 MOI 0.1 -2.3 +1.6 N.D. N.D. -0.1
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