Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS01450 and BPHYT_RS01455 are separated by 244 nucleotidesBPHYT_RS01455 and BPHYT_RS01460 are separated by 31 nucleotidesBPHYT_RS01460 and BPHYT_RS01465 are separated by 114 nucleotidesBPHYT_RS01465 and BPHYT_RS01470 are separated by 103 nucleotides BPHYT_RS01450: BPHYT_RS01450 - DNA-binding protein, at 331,693 to 332,961 _RS01450 BPHYT_RS01455: BPHYT_RS01455 - peptide deformylase, at 333,206 to 333,709 _RS01455 BPHYT_RS01460: BPHYT_RS01460 - methionyl-tRNA formyltransferase, at 333,741 to 334,727 _RS01460 BPHYT_RS01465: BPHYT_RS01465 - lysine transporter LysE, at 334,842 to 335,486 _RS01465 BPHYT_RS01470: BPHYT_RS01470 - protease HtpX homolog, at 335,590 to 336,447 _RS01470
Group Condition BPHYT_RS01450 BPHYT_RS01455 BPHYT_RS01460 BPHYT_RS01465 BPHYT_RS01470
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.1 -5.3 N.D. -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -5.3 N.D. -0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 -5.0 N.D. -0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -5.1 N.D. -0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -5.2 N.D. -0.1 N.D.
carbon source Sebacic (C) -0.1 -4.7 N.D. -0.1 N.D.
carbon source Sebacic (C) -0.1 -4.5 N.D. -0.1 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.1 -4.6 N.D. +0.1 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.0 -4.3 N.D. -0.1 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.0 -4.1 N.D. -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 -4.0 N.D. -0.2 N.D.
nitrogen source L-Isoleucine (N) -0.1 -3.9 N.D. -0.1 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.2 -4.2 N.D. -0.0 N.D.
nitrogen source NAG (N) -0.2 -3.8 N.D. -0.1 N.D.
nitrogen source L-Serine (N) -0.0 -3.9 N.D. +0.0 N.D.
nitrogen source L-Glutamic (N) +0.1 -3.9 N.D. -0.0 N.D.
carbon source Phenylacetic 5 mM (C) -0.2 -3.4 N.D. -0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -4.3 N.D. +0.2 N.D.
carbon source Phenylacetic 5 mM (C) +0.0 -3.6 N.D. +0.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -3.5 N.D. -0.1 N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.1 -2.8 N.D. -0.2 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.3 -2.4 N.D. -0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 -2.8 N.D. -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 -2.6 N.D. +0.0 N.D.
phage P. phyto PHYTO2 MOI 1 -0.2 +2.9 N.D. +0.1 N.D.
stress Paraquat dichloride 0.064 mg/ml +0.0 +2.9 N.D. +0.0 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.2 +2.0 N.D. +1.2 N.D.
phage 24hr PHYTO4 MOI 10 +0.0 +3.6 N.D. -0.2 N.D.
phage P. phyto PHYTO2 MOI 1 +0.0 +4.0 N.D. -0.0 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.1 +3.1 N.D. +1.2 N.D.
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