Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS01185 and BPHYT_RS01190 are separated by 153 nucleotidesBPHYT_RS01190 and BPHYT_RS01195 are separated by 800 nucleotidesBPHYT_RS01195 and BPHYT_RS01200 are separated by 52 nucleotidesBPHYT_RS01200 and BPHYT_RS01205 are separated by 21 nucleotides BPHYT_RS01185: BPHYT_RS01185 - sodium:sulfate symporter, at 271,984 to 273,444 _RS01185 BPHYT_RS01190: BPHYT_RS01190 - hypothetical protein, at 273,598 to 274,338 _RS01190 BPHYT_RS01195: BPHYT_RS01195 - hypothetical protein, at 275,139 to 277,109 _RS01195 BPHYT_RS01200: BPHYT_RS01200 - hypothetical protein, at 277,162 to 279,012 _RS01200 BPHYT_RS01205: BPHYT_RS01205 - hypothetical protein, at 279,034 to 279,222 _RS01205
Group Condition BPHYT_RS01185 BPHYT_RS01190 BPHYT_RS01195 BPHYT_RS01200 BPHYT_RS01205
phage 24hr PHYTO3 MOI 1 -0.0 N.D. -0.1 +0.0 -1.9
phage P. phyto corn MOI 1 -0.3 N.D. +0.0 +0.3 -1.7
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 N.D. +0.1 -0.3 -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 N.D. -0.1 -0.2 -0.9
phage 36hr PHYTO4 MOI 1 -0.2 N.D. +0.1 +0.0 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 N.D. -0.0 -0.2 -1.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.1 N.D. -0.2 -0.3 -0.9
nophagecontrol 36hr Phyto Lib Only -0.1 N.D. -0.3 -0.1 -0.8
phage 24hr PHYTO6 MOI 10 +0.0 N.D. +0.1 -0.2 -1.0
phage 36hr PHYTO10 MOI 10 +0.1 N.D. -0.1 -0.1 -1.0
phage 24hr PHYTO11 MOI 1 -0.3 N.D. +0.0 +0.0 -0.8
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.1 N.D. +0.0 -0.1 -1.0
phage 36hr PHYTO8 MOI 10 +0.1 N.D. -0.1 +0.3 -1.3
phage 36hr PHYTO2 MOI 1 -0.5 N.D. -0.2 +0.2 -0.6
phage 36hr PHYTO10 MOI 1 -0.2 N.D. -0.1 +0.1 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. +0.2 -0.3 -0.7
carbon source Suberic (C) +0.1 N.D. +0.0 +0.1 -1.1
phage 36hr PHYTO9 MOI 1 -0.1 N.D. -0.1 +0.2 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 N.D. +0.3 +0.1 -0.7
phage 36hr PHYTO10 MOI 10 -0.1 N.D. +0.1 +0.2 -0.9
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.2 N.D. +0.3 -0.1 -0.8
motility outer cut, LB soft agar motility assay -0.1 N.D. -0.0 +0.4 -0.6
phage 24hr PHYTO3 MOI 10 +0.1 N.D. +0.1 +0.2 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. -0.3 +0.1 +0.5
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.5 N.D. -0.1 -0.1 +0.7
phage 24hr PHYTO3 MOI 10 -0.2 N.D. +0.0 -0.3 +0.7
carbon source Malonic (C) -0.3 N.D. -0.2 +0.2 +0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. -0.1 +0.7 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.4 N.D. +0.3 +0.0 +0.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 N.D. -0.1 +0.6 +0.6
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BPHYT_RS01185
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BPHYT_RS01190
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BPHYT_RS01200
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BPHYT_RS01205
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