Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS01055 and BPHYT_RS01060 are separated by 79 nucleotidesBPHYT_RS01060 and BPHYT_RS01065 are separated by 161 nucleotidesBPHYT_RS01065 and BPHYT_RS01070 are separated by 48 nucleotidesBPHYT_RS01070 and BPHYT_RS01080 are separated by 3527 nucleotides BPHYT_RS01055: BPHYT_RS01055 - LysR family transcriptional regulator, at 240,736 to 241,665 _RS01055 BPHYT_RS01060: BPHYT_RS01060 - alcohol dehydrogenase, at 241,745 to 242,998 _RS01060 BPHYT_RS01065: BPHYT_RS01065 - GMC family oxidoreductase, at 243,160 to 244,938 _RS01065 BPHYT_RS01070: BPHYT_RS01070 - gluconate 2-dehydrogenase, at 244,987 to 245,706 _RS01070 BPHYT_RS01080: BPHYT_RS01080 - hypothetical protein, at 249,234 to 250,370 _RS01080
Group Condition BPHYT_RS01055 BPHYT_RS01060 BPHYT_RS01065 BPHYT_RS01070 BPHYT_RS01080
phage 24hr PHYTO3 MOI 10 -0.2 -0.2 -0.1 -0.5 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.4 -0.2 -0.3 -0.3 +0.3
nitrogen source L-Lysine (N) -0.6 +0.0 +0.4 -0.4 -0.2
carbon source Sebacic (C) -0.1 +0.3 -0.2 -0.8 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.6 -0.5 -0.1 -0.4 -0.3
phage PHYTO3 MOI 0.1 +0.2 +0.2 -0.5 -0.4 -0.1
carbon source Suberic (C) +0.1 +0.2 -0.5 -0.5 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -0.4 +0.2 -0.2 -0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 +0.1 -0.1 -0.6 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.3 -0.1 -0.2 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -0.2 +0.2 -0.3 -0.4
phage 36hr PHYTO11 MOI 1 -0.1 +0.3 +0.4 -0.4 -0.4
phage 24hr PHYTO7 MOI 1 +0.1 -0.4 +0.4 +0.2 -0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.4 +0.4 +0.1 -0.4 +0.3
phage P. phyto PHYTO2 MOI 0.1 +0.3 -0.1 +0.5 -0.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 -0.2 -0.1 +1.0 -0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.3 -0.2 +0.0 +0.6 +0.2
vitamin With vitamins t1 +0.3 -0.2 -0.3 +0.6 -0.0
phage 36hr PHYTO10 MOI 1 +0.3 -0.1 +0.5 +0.1 -0.4
phage 36hr PHYTO6 MOI 0.1 +0.1 +0.1 +0.5 +0.3 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.1 +0.0 -0.2 -0.1 -0.1
phage 24hr PHYTO6 MOI 1 +0.1 +0.4 +0.3 +0.4 -0.2
nophagecontrol 36hr Phyto Lib Only +0.3 +0.1 +0.1 +0.7 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -0.1 +0.4 +0.5 +0.1
phage 36hr PHYTO2 MOI 1 -0.1 +0.1 +0.6 +0.6 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 +0.2 +0.1 +0.6 -0.0
phage 24hr PHYTO3 MOI 0.1 -0.1 +0.4 +0.4 +0.8 -0.2
phage 24hr PHYTO MOI 0.1 +0.5 +0.3 +0.5 +0.4 -0.3
phage 24hr PHYTO6 MOI 10 +0.1 +0.4 +1.0 +0.4 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 +0.0 +0.6 +1.0 +0.0
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