Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS00880 and BPHYT_RS00885 are separated by 46 nucleotidesBPHYT_RS00885 and BPHYT_RS00890 are separated by 724 nucleotidesBPHYT_RS00890 and BPHYT_RS00895 are separated by 236 nucleotidesBPHYT_RS00895 and BPHYT_RS00900 are separated by 38 nucleotides BPHYT_RS00880: BPHYT_RS00880 - hypothetical protein, at 201,106 to 202,275 _RS00880 BPHYT_RS00885: BPHYT_RS00885 - chemotaxis protein CheY, at 202,322 to 203,386 _RS00885 BPHYT_RS00890: BPHYT_RS00890 - membrane protein, at 204,111 to 205,199 _RS00890 BPHYT_RS00895: BPHYT_RS00895 - MFS transporter, at 205,436 to 206,818 _RS00895 BPHYT_RS00900: BPHYT_RS00900 - beta-lactamase, at 206,857 to 207,183 _RS00900
Group Condition BPHYT_RS00880 BPHYT_RS00885 BPHYT_RS00890 BPHYT_RS00895 BPHYT_RS00900
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 -1.8 -0.3 -0.1 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.6 -0.2 -0.2 -1.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.0 -1.1 +0.1 -0.0 -1.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.1 -1.3 -0.1 +0.1 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.6 -0.7 -0.3 -0.5
carbon source succinate (C) -0.4 -1.0 +0.0 +0.4 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.3 -1.6 +0.2 -0.2 +0.4
phage 24hr PHYTO7 MOI 10 +0.0 +0.1 -0.3 +0.6 -1.5
phage 24hr PHYTO6 MOI 0.1 +0.1 -1.5 -0.1 +0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 -1.2 +0.0 +0.6 -0.2
phage 24hr PHYTO3 MOI 1 -0.0 -1.8 +0.0 +0.5 +0.4
phage 24hr PHYTO11 MOI 0.1 +0.2 -1.5 -0.2 +0.9 -0.2
phage 24hr PHYTO4 MOI 10 -0.1 -2.3 +0.1 +0.7 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 -0.6 +0.4 +0.5 -0.8
carbon source Dodecandioic (C) +0.2 +0.7 -0.0 +0.4 -1.5
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.5 -0.9 +0.3 -0.3 +1.2
phage 36hr PHYTO4 MOI 1 +0.1 -1.5 -0.0 +0.8 +0.6
phage 24hr PHYTO3 MOI 10 +0.1 -0.5 -0.3 -0.5 +1.4
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.4 -0.7 +0.3 +0.5 -0.4
phage 36hr PHYTO6 MOI 10 +0.2 -1.3 +0.3 +0.9 +0.1
phage 36hr PHYTO MOI 0.1 -0.1 -1.0 +0.1 +0.5 +0.7
phage 24hr PHYTO10 MOI 10 -0.1 -0.9 +0.2 +0.4 +0.9
phage 24hr PHYTO6 MOI 10 +0.0 -0.8 -0.4 +0.5 +1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.0 +0.9 +0.3 -0.4 +0.7
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.2 +1.1 -0.4 +0.0 +0.7
carbon source Tween 20 (C) +0.0 +1.6 -0.3 +0.1 +0.3
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.3 +2.3 +0.1 -0.1 -1.0
phage 24hr PHYTO2 MOI 0.1 +0.1 +0.6 -0.3 +0.3 +1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.3 +0.1 +0.7 +0.8
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.4 +2.9 -0.0 -0.0 +0.5
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