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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS00880 and BPHYT_RS00885 are separated by 46 nucleotides
BPHYT_RS00885 and BPHYT_RS00890 are separated by 724 nucleotides
BPHYT_RS00890 and BPHYT_RS00895 are separated by 236 nucleotides
BPHYT_RS00895 and BPHYT_RS00900 are separated by 38 nucleotides
BPHYT_RS00880: BPHYT_RS00880 - hypothetical protein, at 201,106 to 202,275
_RS00880
BPHYT_RS00885: BPHYT_RS00885 - chemotaxis protein CheY, at 202,322 to 203,386
_RS00885
BPHYT_RS00890: BPHYT_RS00890 - membrane protein, at 204,111 to 205,199
_RS00890
BPHYT_RS00895: BPHYT_RS00895 - MFS transporter, at 205,436 to 206,818
_RS00895
BPHYT_RS00900: BPHYT_RS00900 - beta-lactamase, at 206,857 to 207,183
_RS00900
Group
Condition
BPHYT
_RS00880
BPHYT
_RS00885
BPHYT
_RS00890
BPHYT
_RS00895
BPHYT
_RS00900
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
-0.1
-1.8
-0.3
-0.1
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
-0.6
-0.2
-0.2
-1.1
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.0
-1.1
+0.1
-0.0
-1.0
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
+0.1
-1.3
-0.1
+0.1
-0.8
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-0.6
-0.7
-0.3
-0.5
carbon source
succinate (C)
-0.4
-1.0
+0.0
+0.4
-0.6
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
-0.3
-1.6
+0.2
-0.2
+0.4
phage
24hr PHYTO7 MOI 10
+0.0
+0.1
-0.3
+0.6
-1.5
phage
24hr PHYTO6 MOI 0.1
+0.1
-1.5
-0.1
+0.5
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.2
-1.2
+0.0
+0.6
-0.2
phage
24hr PHYTO3 MOI 1
-0.0
-1.8
+0.0
+0.5
+0.4
phage
24hr PHYTO11 MOI 0.1
+0.2
-1.5
-0.2
+0.9
-0.2
phage
24hr PHYTO4 MOI 10
-0.1
-2.3
+0.1
+0.7
+1.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.1
-0.6
+0.4
+0.5
-0.8
carbon source
Dodecandioic (C)
+0.2
+0.7
-0.0
+0.4
-1.5
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-0.5
-0.9
+0.3
-0.3
+1.2
phage
36hr PHYTO4 MOI 1
+0.1
-1.5
-0.0
+0.8
+0.6
phage
24hr PHYTO3 MOI 10
+0.1
-0.5
-0.3
-0.5
+1.4
in planta
Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
+0.4
-0.7
+0.3
+0.5
-0.4
phage
36hr PHYTO6 MOI 10
+0.2
-1.3
+0.3
+0.9
+0.1
phage
36hr PHYTO MOI 0.1
-0.1
-1.0
+0.1
+0.5
+0.7
phage
24hr PHYTO10 MOI 10
-0.1
-0.9
+0.2
+0.4
+0.9
phage
24hr PHYTO6 MOI 10
+0.0
-0.8
-0.4
+0.5
+1.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.0
+0.9
+0.3
-0.4
+0.7
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days
+0.2
+1.1
-0.4
+0.0
+0.7
carbon source
Tween 20 (C)
+0.0
+1.6
-0.3
+0.1
+0.3
in planta
Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days
+0.3
+2.3
+0.1
-0.1
-1.0
phage
24hr PHYTO2 MOI 0.1
+0.1
+0.6
-0.3
+0.3
+1.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.7
-0.3
+0.1
+0.7
+0.8
in planta
Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days
+0.4
+2.9
-0.0
-0.0
+0.5
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