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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS00790 and BPHYT_RS00795 are separated by 189 nucleotides
BPHYT_RS00795 and BPHYT_RS00800 are separated by 14 nucleotides
BPHYT_RS00800 and BPHYT_RS00805 overlap by 8 nucleotides
BPHYT_RS00805 and BPHYT_RS00810 overlap by 4 nucleotides
BPHYT_RS00790: BPHYT_RS00790 - conjugal transfer protein TraF, at 179,432 to 180,142
_RS00790
BPHYT_RS00795: BPHYT_RS00795 - conjugal transfer protein TraJ, at 180,332 to 181,090
_RS00795
BPHYT_RS00800: BPHYT_RS00800 - Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9), at 181,105 to 181,932
_RS00800
BPHYT_RS00805: BPHYT_RS00805 - type IV secretion system protein VirB10, at 181,925 to 183,127
_RS00805
BPHYT_RS00810: BPHYT_RS00810 - type IV secretion system protein VirB11, at 183,124 to 184,251
_RS00810
Group
Condition
BPHYT
_RS00790
BPHYT
_RS00795
BPHYT
_RS00800
BPHYT
_RS00805
BPHYT
_RS00810
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-0.8
-1.0
-0.1
-0.2
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-2.0
+0.6
-0.1
+0.1
-0.3
nitrogen source
Ammonium chloride (N)
-1.3
-0.3
-0.2
+0.1
-0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
-0.9
-0.4
-0.1
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.3
+0.3
+0.1
-0.2
-0.3
carbon source
D-Galacturonic Acid (C)
-1.1
-0.3
+0.2
-0.3
+0.1
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days
-0.5
-0.5
+0.0
+0.3
-0.5
phage
36hr PHYTO3 MOI 0.1
-1.0
-0.6
-0.1
+0.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.6
+0.3
-0.3
-0.4
-0.2
phage
24hr PHYTO7 MOI 10
-0.6
+0.2
+0.3
+0.4
-0.4
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.9
-0.1
+0.1
+0.6
+0.3
carbon source
Protocatechuic Acid (C)
+0.9
+0.3
+0.1
-0.6
-0.7
in planta
Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1
+0.8
-0.7
-0.2
+0.2
-0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.7
-0.5
-0.3
+0.5
-0.1
carbon source
Tween 20 (C)
+0.9
-0.3
-0.1
-0.4
+0.3
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days
+0.9
-0.2
+0.2
+0.4
-0.4
motility
outer cut, LB soft agar motility assay
+1.3
-0.2
+0.3
-0.2
-0.2
carbon source
2-Deoxyadenosine 5-monophosphate 5 mM (C)
+1.0
+0.2
-0.4
+0.0
+0.2
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
+1.1
+0.1
+0.3
+0.3
-0.6
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
+1.2
+0.2
-0.4
+0.2
+0.0
carbon source
Sebacic (C)
+1.0
-0.2
+0.2
+0.1
+0.2
tn7 efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
+1.0
+0.2
+0.1
-0.3
+0.3
in planta
Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days
+0.8
+0.2
-0.1
+0.8
-0.5
carbon source
D-Glucose 20 mM (C)
+1.5
-0.2
+0.0
+0.0
+0.1
phage
24hr PHYTO6 MOI 1
+1.0
+0.4
-0.3
-0.1
+0.3
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
+1.3
-0.3
+0.1
+0.0
+0.3
carbon source
Phenylacetic 5 mM (C)
+1.0
+0.5
+0.3
-0.2
-0.0
vitamin
With vitamins t2
+1.0
-0.1
+0.1
+0.2
+0.4
carbon source
Phenylacetic 5 mM (C)
+1.7
+0.1
-0.2
+0.1
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.7
+0.5
+0.2
+0.8
-0.1
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