Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS00745 and BPHYT_RS00750 overlap by 4 nucleotidesBPHYT_RS00750 and BPHYT_RS00755 are separated by 526 nucleotidesBPHYT_RS00755 and BPHYT_RS00760 overlap by 8 nucleotidesBPHYT_RS00760 and BPHYT_RS00765 overlap by 4 nucleotides BPHYT_RS00745: BPHYT_RS00745 - transcriptional regulator, at 171,763 to 172,167 _RS00745 BPHYT_RS00750: BPHYT_RS00750 - SpoVT/AbrB domain-containing protein, at 172,164 to 172,436 _RS00750 BPHYT_RS00755: BPHYT_RS00755 - cobyric acid synthase CobQ, at 172,963 to 173,835 _RS00755 BPHYT_RS00760: BPHYT_RS00760 - hypothetical protein, at 173,828 to 174,412 _RS00760 BPHYT_RS00765: BPHYT_RS00765 - lytic transglycosylase, at 174,409 to 175,086 _RS00765
Group Condition BPHYT_RS00745 BPHYT_RS00750 BPHYT_RS00755 BPHYT_RS00760 BPHYT_RS00765
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.8 -0.4 -0.1 +0.0
phage 36hr PHYTO11 MOI 0.1 N.D. -0.6 +0.0 -0.4 -0.3
carbon source Dodecandioic (C) N.D. -0.6 +0.1 -0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.1 -0.1 +0.1 +0.2
nitrogen source Uridine (N) N.D. -0.9 -0.2 +0.2 +0.1
carbon source Protocatechuic Acid (C) N.D. -0.7 -0.2 +0.3 -0.2
r2a control R2A control N.D. -0.8 -0.3 +0.5 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.7 +0.4 +0.1 -0.3
phage PHYTO3 MOI 0.1 N.D. +0.5 -0.6 +0.1 -0.5
nitrogen source L-Lysine (N) N.D. -0.7 +0.4 +0.1 -0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. -1.0 +0.2 +0.1 +0.3
phage P. phyto PHYTO2 MOI 1 N.D. +0.1 -0.3 +0.5 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.7 -0.3 -0.2 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.5 -0.2 +0.5 +0.2
phage 24hr PHYTO6 MOI 1 N.D. +0.8 -0.2 +0.0 -0.6
nitrogen source L-Asparagine (N) N.D. -0.6 +0.3 +0.5 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.5 +0.6 +0.4 -0.2
phage 36hr PHYTO6 MOI 0.1 N.D. +1.0 +0.2 -0.6 -0.3
vitamin With vitamins t1 N.D. +0.6 +0.3 -0.6 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.7 +0.2 -0.3 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +1.0 -0.0 -0.2 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.8 +0.1 +0.1 -0.3
motility inner cut, LB soft agar motility assay N.D. +1.2 -0.1 +0.1 -0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.0 -0.3 +0.8 +0.3
carbon source Malonic (C) N.D. +1.1 -0.3 +0.3 +0.0
nitrogen source Ammonium chloride (N) N.D. +0.7 +0.2 +0.5 -0.2
motility outer cut, LB soft agar motility assay N.D. +1.1 +0.3 +0.0 -0.2
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. +0.4 +0.1 +0.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.9 +0.4 -0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 +0.4 +0.6 +0.3
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BPHYT_RS00745
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BPHYT_RS00750
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BPHYT_RS00765
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