Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS00385 and BPHYT_RS00390 are separated by 229 nucleotidesBPHYT_RS00390 and BPHYT_RS00395 are separated by 259 nucleotidesBPHYT_RS00395 and BPHYT_RS00400 are separated by 510 nucleotidesBPHYT_RS00400 and BPHYT_RS00405 are separated by 17 nucleotides BPHYT_RS00385: BPHYT_RS00385 - decarboxylase, at 96,224 to 97,567 _RS00385 BPHYT_RS00390: BPHYT_RS00390 - AraC family transcriptional regulator, at 97,797 to 98,831 _RS00390 BPHYT_RS00395: BPHYT_RS00395 - aldehyde dehydrogenase, at 99,091 to 100,518 _RS00395 BPHYT_RS00400: BPHYT_RS00400 - hypothetical protein, at 101,029 to 101,316 _RS00400 BPHYT_RS00405: BPHYT_RS00405 - hypothetical protein, at 101,334 to 101,687 _RS00405
Group Condition BPHYT_RS00385 BPHYT_RS00390 BPHYT_RS00395 BPHYT_RS00400 BPHYT_RS00405
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.2 -0.2 -0.5 -0.4
carbon source Phenylacetic 5 mM (C) +0.0 -0.5 -0.1 -0.9 +0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.3 -0.5 +0.3 -0.6 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 -0.3 +0.1 -0.5 -0.5
nophagecontrol 24hr Phyto Lib Only -0.1 +0.3 -0.0 -0.8 -0.0
nophagecontrol 36hr Phyto Lib only -0.4 +0.3 +0.1 -0.5 -0.1
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.1 +0.2 -0.4 -0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 -0.4 +0.3 -0.4 +0.1
phage 36hr PHYTO7 MOI 1 -0.3 +0.2 +0.1 -0.8 +0.2
nophagecontrol 24hr Phyto Lib Only -0.1 +0.3 +0.0 -0.8 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.4 -0.4 -0.4 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.0 +0.3 +0.2 -0.9 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.0 -0.4 +0.5 -0.2
nophagecontrol 36hr Phyto Lib Only -0.2 +0.5 -0.2 -0.6 +0.1
phage 36hr PHYTO11 MOI 1 -0.1 +0.2 +0.3 -0.7 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.2 -0.5 +0.4 +0.3
phage 24hr PHYTO2 MOI 0.1 -0.1 +0.6 -0.4 -0.4 -0.0
phage 24hr PHYTO10 MOI 10 -0.3 +0.4 +0.1 -0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 +0.2 -0.7 +0.4 -0.0
phage 24hr PHYTO10 MOI 10 -0.3 +0.4 +0.3 -0.8 +0.2
phage 24hr PHYTO6 MOI 1 -0.3 +0.4 +0.3 -0.5 -0.0
motility inner cut, LB soft agar motility assay +0.0 +0.1 +0.4 -0.8 +0.1
phage 36hr PHYTO2 MOI 1 -0.0 +0.5 +0.1 -0.7 +0.1
phage 24hr PHYTO3 MOI 10 -0.2 +0.5 +0.2 -0.4 +0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.2 +0.1 +0.5 -0.6 +0.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.2 -0.5 +0.2 +0.5 +0.2
carbon source Protocatechuic Acid (C) -0.1 -0.1 +0.4 +0.9 +0.1
carbon source adipate (C) +0.1 -0.2 +0.2 +1.1 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.5 +0.2 +0.6 +0.1
motility outer cut, LB soft agar motility assay +0.1 +0.1 -0.0 +1.2 +0.1
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