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Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS00120 and BPHYT_RS00125 are separated by 334 nucleotides
BPHYT_RS00125 and BPHYT_RS00130 are separated by 43 nucleotides
BPHYT_RS00130 and BPHYT_RS00135 are separated by 93 nucleotides
BPHYT_RS00135 and BPHYT_RS00140 are separated by 49 nucleotides
BPHYT_RS00120: BPHYT_RS00120 - aldehyde dehydrogenase, at 34,086 to 35,606
_RS00120
BPHYT_RS00125: BPHYT_RS00125 - AraC family transcriptional regulator, at 35,941 to 36,990
_RS00125
BPHYT_RS00130: BPHYT_RS00130 - ATPase P, at 37,034 to 39,139
_RS00130
BPHYT_RS00135: BPHYT_RS00135 - hypothetical protein, at 39,233 to 39,436
_RS00135
BPHYT_RS00140: BPHYT_RS00140 - MerR family transcriptional regulator, at 39,486 to 39,917
_RS00140
Group
Condition
BPHYT
_RS00120
BPHYT
_RS00125
BPHYT
_RS00130
BPHYT
_RS00135
BPHYT
_RS00140
stress
Zinc 0.5 mM
-0.1
+0.1
-6.3
N.D.
-4.1
r2a control
R2A control
-0.0
-0.5
-0.3
N.D.
-0.8
carbon source
2-Deoxy-D-ribonic lithium salt (C)
-1.0
-0.3
+0.2
N.D.
-0.5
r2a control
R2A control
-0.3
-0.4
-0.3
N.D.
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.4
-0.2
-0.3
N.D.
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.1
+0.1
-0.2
N.D.
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.3
-0.3
-0.5
N.D.
-0.2
nitrogen source
L-Phenylalanine (N)
-0.5
-0.2
-0.0
N.D.
-0.5
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
-0.2
-0.3
-0.6
N.D.
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.0
-0.5
-0.4
N.D.
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.5
-0.5
-0.1
N.D.
-0.1
phage
36hr PHYTO7 MOI 0.1
-0.3
-0.2
-0.2
N.D.
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days
-0.7
-0.2
-0.2
N.D.
+0.0
motility
outer cut, LB soft agar motility assay
+0.2
-0.2
-0.3
N.D.
-0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.6
-0.5
+0.1
N.D.
+0.2
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
-1.0
+0.2
-0.1
N.D.
+0.1
motility
outer cut, LB soft agar motility assay
+0.0
+0.2
-0.2
N.D.
-0.8
in planta
Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.5
+0.3
+0.3
N.D.
-0.3
in planta
Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1
-0.8
+0.2
+0.2
N.D.
+0.2
phage
36hr PHYTO8 MOI 0.1
+0.0
-0.5
-0.3
N.D.
+0.4
phage
P. phyto PHYTO2 MOI 0.1
-0.3
-0.1
-0.4
N.D.
+0.4
motility
inner cut, LB soft agar motility assay
+0.2
+0.3
-0.2
N.D.
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.1
-0.4
-0.3
N.D.
+0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.4
+0.1
-0.6
N.D.
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.5
-0.0
+0.4
N.D.
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.3
+0.4
+0.5
N.D.
+0.5
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB
+0.1
+0.3
+0.3
N.D.
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.3
-0.3
+0.6
N.D.
+0.9
stress
Nickel (II) chloride 1 mM
+0.2
+0.1
+0.5
N.D.
+0.7
stress
Cobalt chloride 0.25 mM
-0.1
+0.3
+0.8
N.D.
+0.8
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