Fitness for 5 genes in Acidovorax sp. GW101-3H11

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500 ntAc3H11_2215 and Ac3H11_2216 are separated by 51 nucleotidesAc3H11_2216 and Ac3H11_2217 overlap by 4 nucleotidesAc3H11_2217 and Ac3H11_2218 are separated by 124 nucleotidesAc3H11_2218 and Ac3H11_2219 are separated by 4 nucleotides Ac3H11_2215 - Phosphomannomutase (EC 5.4.2.8), at 447,542 to 448,930 _2215 Ac3H11_2216 - Hydrolase, carbon-nitrogen family, at 448,982 to 449,779 _2216 Ac3H11_2217 - Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase (EC 2.4.99.12) [often with (EC 2.4.99.13) also], at 449,776 to 451,143 _2217 Ac3H11_2218 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 451,268 to 453,568 _2218 Ac3H11_2219 - Type I secretion outer membrane protein, TolC precursor, at 453,573 to 454,907 _2219
Group Condition Ac3H11_2215 Ac3H11_2216 Ac3H11_2217 Ac3H11_2218 Ac3H11_2219
nitrogen source L-Alanine (N) N.D. -0.1 N.D. -0.1 -3.6
nitrogen source Ammonium chloride (N) N.D. +0.6 N.D. +0.1 -3.1
stress nitrate 45 mM N.D. -0.2 N.D. +0.1 -1.0
nitrogen source Uridine (N) N.D. -0.4 N.D. +0.0 -0.7
stress copper (II) chloride 2 mM N.D. -0.5 N.D. -0.2 -0.3
carbon source L-Lactate (C) N.D. -0.6 N.D. +0.1 -0.5
nitrogen source L-Threonine (N) N.D. +0.7 N.D. +0.1 -1.6
motility inner cut, R2A soft agar motility assay N.D. +0.1 N.D. +0.0 -0.9
lb LB N.D. +0.2 N.D. -0.0 -0.8
nitrogen source L-Isoleucine (N) N.D. +0.1 N.D. +0.1 -0.7
carbon source a-Ketoglutaric (C) N.D. -0.8 N.D. +0.1 +0.3
stress nitrate 100 mM N.D. +0.3 N.D. +0.0 -0.8
stress D-Cycloserine 0.05 mg/ml N.D. -0.1 N.D. +0.2 -0.6
stress Cisplatin 0.0025 mg/ml N.D. -0.5 N.D. -0.1 +0.4
stress Phosphomycin 0.02 mg/ml N.D. -0.5 N.D. +0.2 +0.1
carbon source L-Malic (C) N.D. -0.6 N.D. +0.1 +0.5
phage phage 93.2 N.D. -0.6 N.D. +0.2 +0.6
stress Cisplatin 0.005 mg/ml N.D. +0.5 N.D. +0.5 -0.8
nitrogen source L-Leucine (N) N.D. +0.6 N.D. +0.2 -0.4
stress Cobalt chloride 0.32 mM N.D. -0.3 N.D. +0.1 +0.5
pH LB, pH10 N.D. +0.5 N.D. +0.3 -0.4
pH LB, pH10 N.D. +0.3 N.D. +0.4 -0.2
lb LB N.D. +0.2 N.D. -0.2 +0.5
lb LB N.D. -0.3 N.D. +0.0 +0.9
nitrogen source L-tyrosine (N) N.D. +0.0 N.D. +0.2 +0.7
carbon source D-Mannose (C) N.D. +0.2 N.D. +0.2 +0.5
stress nitrate 20 mM N.D. +0.4 N.D. +0.2 +0.4
carbon source D-Galactose (C) N.D. +0.3 N.D. +0.0 +0.8
stress Bacitracin 0.008 mg/ml N.D. +0.3 N.D. +0.1 +0.8
stress Nickel (II) chloride 0.8 mM N.D. +0.4 N.D. +0.2 +0.6
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