Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS33685 and AZOBR_RS33690 overlap by 1 nucleotidesAZOBR_RS33690 and AZOBR_RS33695 are separated by 79 nucleotidesAZOBR_RS33695 and AZOBR_RS33700 are separated by 397 nucleotidesAZOBR_RS33700 and AZOBR_RS33705 are separated by 249 nucleotides AZOBR_RS33685: AZOBR_RS33685 - membrane protein, at 86,800 to 87,222 _RS33685 AZOBR_RS33690: AZOBR_RS33690 - hypothetical protein, at 87,222 to 88,724 _RS33690 AZOBR_RS33695: AZOBR_RS33695 - 4-hydroxy-4-methyl-2-oxoglutarate aldolase, at 88,804 to 89,472 _RS33695 AZOBR_RS33700: AZOBR_RS33700 - tRNA-Gly, at 89,870 to 89,944 _RS33700 AZOBR_RS33705: AZOBR_RS33705 - flagellin modification protein FlmA, at 90,194 to 91,186 _RS33705
Group Condition AZOBR_RS33685 AZOBR_RS33690 AZOBR_RS33695 AZOBR_RS33700 AZOBR_RS33705
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.1 +0.4 +0.1 -8.7
stress 2-pentyl-furan 1.335528 mM N.D. +0.1 -0.1 -0.2 -7.1
stress 2-pentyl-furan 1.335528 mM N.D. +0.0 -0.1 -0.3 -6.7
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.4 +0.0 +0.3 -7.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.4 +0.7 -0.1 -7.3
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.1 +0.5 -0.3 -5.8
stress 2-pentyl-furan 1.780666667 mM N.D. -0.1 -0.1 -0.1 -4.9
stress 2-pentyl-furan 1.335528 mM N.D. -0.2 +0.1 -0.3 -4.3
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.2 +0.3 +0.1 -4.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.2 +0.4 +0.2 -4.6
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.2 +0.5 +0.3 -4.6
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. -0.0 +0.2 +0.2 -3.7
lb LB N.D. +0.1 +0.0 -0.0 -3.0
stress 2-pentyl-furan 1.780666667 mM N.D. -0.3 -0.1 +0.2 -2.7
stress 2-pentyl-furan 1.335528 mM N.D. +0.0 -0.3 -0.5 -2.0
stress 2-pentyl-furan 2.671 mM N.D. -0.2 +0.3 -0.3 -2.3
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.1 +0.5 -0.0 -3.0
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. +0.2 -0.0 +0.3 -2.7
stress 2-pentyl-furan 1.780666667 mM N.D. -0.0 +0.2 -0.4 -1.9
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.3 +0.8 +0.1 -3.3
nitrogen source casaminos (N) N.D. +0.2 -0.3 +0.4 -1.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.2 +0.4 -1.0 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 +0.4 -0.5 +1.1
nitrogen source L-Isoleucine (N) N.D. +0.2 -0.9 +0.3 +1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 +0.7 -0.8 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 -0.6 -0.0 +1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.6 +0.7 -0.4 +1.3
motility inner cut, LB soft agar motility assay N.D. -0.3 +0.0 +1.0 +2.2
motility inner cut, LB soft agar motility assay N.D. +0.1 -0.2 +0.2 +2.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.1 +0.9 +1.3 +2.4
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