Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS32320 and AZOBR_RS32325 are separated by 257 nucleotidesAZOBR_RS32325 and AZOBR_RS32335 are separated by 995 nucleotidesAZOBR_RS32335 and AZOBR_RS29645 are separated by 90 nucleotidesAZOBR_RS29645 and AZOBR_RS32340 are separated by 150 nucleotides AZOBR_RS32320: AZOBR_RS32320 - LacI family transcriptional regulator, at 617,826 to 618,818 _RS32320 AZOBR_RS32325: AZOBR_RS32325 - Fructose PTS system (E-I, HPr, and E-IIA components) (from data), at 619,076 to 621,616 _RS32325 AZOBR_RS32335: AZOBR_RS32335 - fructose-specific PTS system (fructose 1-phosphate forming), EII-B'BC components (FruA) (from data), at 622,612 to 624,354 _RS32335 AZOBR_RS29645: AZOBR_RS29645 - suhB, at 624,445 to 624,522 _RS29645 AZOBR_RS32340: AZOBR_RS32340 - hypothetical protein, at 624,673 to 625,173 _RS32340
Group Condition AZOBR_RS32320 AZOBR_RS32325 AZOBR_RS32335 AZOBR_RS29645 AZOBR_RS32340
nitrogen source L-Isoleucine (N) -2.0 -0.4 -0.2 -2.5 -1.2
carbon source D-Fructose (C) -0.7 -2.5 -2.2 -0.5 +0.3
stress 2-pentyl-furan 1.780666667 mM +0.2 -0.3 -0.1 -2.9 -2.4
carbon source D-Fructose (C) -0.6 -2.4 -2.3 -0.0 +0.2
stress 2-pentyl-furan 1.780666667 mM +0.4 -0.2 +0.0 -3.0 -2.1
stress 1-octen-3-ol 0.7753276667 mM -0.4 +0.2 -0.1 -2.8 -1.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -4.4 -0.9 -0.4 +0.3 +1.0
stress 2-pentyl-furan 1.335528 mM +0.6 -0.6 +0.2 -2.9 -1.5
stress 2-pentyl-furan 1.335528 mM -0.1 +0.1 +0.1 -2.9 -1.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.7 -0.8 -0.4 -0.9 -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -3.1 -1.0 -0.2 +0.8 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -2.0 -0.5 -0.7 +0.1 -0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.9 -0.4 -0.8 -0.5 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.6 +0.2 -0.2 -1.2 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.7 -0.3 +0.1 -0.2 -0.3
lb no stress control LB no stress control +0.2 -0.3 -0.1 -2.8 -0.3
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM -0.4 +0.2 -0.1 -2.9 +0.1
stress 2-pentyl-furan 1.780666667 mM +0.3 -0.2 -0.1 -2.9 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.6 +0.5 +0.3 -0.8 -0.9
stress 3-octanone 6.3476 mM +0.6 -0.2 -0.1 -2.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.3 +0.5 +0.2 -1.0 +0.6
stress 2-pentyl-furan 2.671 mM -0.3 -0.2 +0.0 -2.8 +1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.5 +0.6 +0.1 +0.3 +0.2
stress Benzethonium chloride 0.003 mM +0.9 -0.6 -1.0 -0.9 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days -2.2 +0.5 +0.5 +0.1 +0.5
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days -1.8 +0.3 +0.5 +0.6 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.7 +0.4 -0.2 +1.3 +0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -1.7 +0.4 +1.1 -0.2 +0.7
motility inner cut, LB soft agar motility assay +1.4 -1.0 -0.0 +0.9 -0.2
motility inner cut, LB soft agar motility assay +2.5 +0.1 -0.2 +1.5 +0.1
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