Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS31680 and AZOBR_RS31685 are separated by 39 nucleotidesAZOBR_RS31685 and AZOBR_RS31690 are separated by 636 nucleotidesAZOBR_RS31690 and AZOBR_RS31700 are separated by 1412 nucleotidesAZOBR_RS31700 and AZOBR_RS31705 are separated by 32 nucleotides AZOBR_RS31680: AZOBR_RS31680 - diguanylate cyclase, at 475,500 to 476,516 _RS31680 AZOBR_RS31685: AZOBR_RS31685 - hypothetical protein, at 476,556 to 477,035 _RS31685 AZOBR_RS31690: AZOBR_RS31690 - lactate dehydrogenase, at 477,672 to 478,655 _RS31690 AZOBR_RS31700: AZOBR_RS31700 - gamma-glutamyltransferase, at 480,068 to 481,651 _RS31700 AZOBR_RS31705: AZOBR_RS31705 - hypothetical protein, at 481,684 to 481,941 _RS31705
Group Condition AZOBR_RS31680 AZOBR_RS31685 AZOBR_RS31690 AZOBR_RS31700 AZOBR_RS31705
stress Benzethonium chloride 0.003 mM -0.1 -0.8 -2.4 -0.1 -0.1
stress 2-pentyl-furan 1.780666667 mM -1.7 -0.1 -1.2 -0.0 -0.4
stress 2-pentyl-furan 1.335528 mM -1.3 -0.1 -0.8 -0.1 -1.2
stress 2-pentyl-furan 2.671 mM +0.0 -3.1 -1.0 +0.4 +0.3
stress 2-pentyl-furan 1.780666667 mM -1.5 -0.1 -0.6 +0.2 -1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.1 -4.6 +1.2 -0.8 +0.2
stress 2-pentyl-furan 1.335528 mM -1.1 -0.6 -0.6 +0.2 -0.5
nitrogen source L-Histidine (N) +0.2 -0.8 -1.5 +0.1 -0.6
stress 2-pentyl-furan 1.780666667 mM -1.9 +0.1 -0.5 +0.2 +0.1
motility outer cut, LB soft agar motility assay +1.1 -1.3 -1.3 -0.2 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.5 -4.6 +0.9 +0.0 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.4 -1.3 -2.0 -0.2 +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 -1.5 +0.7 +0.3 -0.6
stress 2-pentyl-furan 1.335528 mM -0.7 +0.3 +0.7 -0.1 -1.0
nitrogen source L-Isoleucine (N) +0.1 -1.7 +0.7 -0.6 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 -0.1 -0.4 +0.3 -1.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.3 -1.8 +0.8 +0.4 +0.6
carbon source D-Gluconic Acid (C) +0.3 -0.6 -0.8 +0.3 +1.0
motility inner cut, LB soft agar motility assay +0.2 +0.8 +0.4 +0.1 -1.3
carbon source D,L-Lactate (C) +0.2 -0.7 -0.4 +0.4 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.2 +0.2 -1.1 +0.4 +0.0
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days +0.1 +0.8 +0.8 +0.1 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 +0.2 -0.5 -0.2 +0.8
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.1 +0.6 +1.2 +0.3 -0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 +0.1 -0.4 +0.0 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.2 -1.3 +1.4 -0.4 +0.7
stress D-Cycloserine 0.125 mg/ml -0.1 +1.6 -0.4 +1.0 -0.4
motility inner cut, LB soft agar motility assay +0.2 +1.9 -0.4 +0.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 -1.0 +0.4 +0.1 +1.4
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.0 +1.3 +1.1 +0.6 +0.3
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