Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS31455 and AZOBR_RS31460 are separated by 27 nucleotidesAZOBR_RS31460 and AZOBR_RS31465 are separated by 110 nucleotidesAZOBR_RS31465 and AZOBR_RS31470 are separated by 13 nucleotidesAZOBR_RS31470 and AZOBR_RS31475 are separated by 38 nucleotides AZOBR_RS31455: AZOBR_RS31455 - C4-dicarboxylate ABC transporter, at 424,890 to 425,378 _RS31455 AZOBR_RS31460: AZOBR_RS31460 - tripartite tricarboxylate transporter TctA, at 425,406 to 426,878 _RS31460 AZOBR_RS31465: AZOBR_RS31465 - membrane protein, at 426,989 to 427,657 _RS31465 AZOBR_RS31470: AZOBR_RS31470 - universal stress protein UspA, at 427,671 to 428,549 _RS31470 AZOBR_RS31475: AZOBR_RS31475 - chemotaxis protein CheY, at 428,588 to 429,262 _RS31475
Group Condition AZOBR_RS31455 AZOBR_RS31460 AZOBR_RS31465 AZOBR_RS31470 AZOBR_RS31475
stress Benzethonium chloride 0.003 mM N.D. -0.4 -0.3 +0.3 -0.7
motility outer cut, LB soft agar motility assay N.D. +0.3 +0.1 -0.9 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 -0.4 -0.6 +0.3
carbon source L-Leucine (C) N.D. -0.2 +0.0 -0.7 +0.3
motility inner cut, LB soft agar motility assay N.D. -0.3 -0.4 +0.3 -0.1
stress 3-octanone 5.2896 mM N.D. -0.3 +0.3 -0.4 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.3 -0.3 -0.1 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.1 +0.2 -0.3 +0.5
stress 2-pentyl-furan 1.780666667 mM N.D. +0.1 +0.5 -0.6 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.2 +0.6 +0.6 -0.1
anaerobic; rich media LB; anaerobic N.D. +0.1 +0.2 +0.6 -0.1
stress 3-octanone 6.3476 mM N.D. +0.1 +0.3 +0.4 +0.2
nitrogen source Glucuronamide (N) N.D. +0.2 +0.2 +0.3 +0.3
nitrogen source L-Phenylalanine (N) N.D. +0.0 +0.1 +0.7 +0.3
carbon source D-Gluconic Acid (C) N.D. +0.2 +0.2 +0.4 +0.2
stress Paraquat dichloride 0.04 mg/ml N.D. +0.1 -0.2 +1.1 +0.1
carbon source L-Malic (C) N.D. +0.0 +0.5 +0.3 +0.3
stress 2-pentyl-furan 2.671 mM N.D. +0.1 +0.3 +0.3 +0.4
nitrogen source Cytosine (N) N.D. +0.3 +0.2 +0.5 +0.3
stress 3-octanone 6.3476 mM N.D. +0.2 +0.4 +0.2 +0.3
lb LB N.D. +0.3 +0.2 +0.7 +0.1
nitrogen source L-Glutamic (N) N.D. +0.5 -0.0 +0.6 +0.2
stress Sisomicin 0.01 mg/ml N.D. -0.0 +0.4 +0.8 +0.1
nitrogen source L-Threonine (N) N.D. -0.1 +0.4 +0.8 +0.3
carbon source L-Proline (C) N.D. +0.3 +0.3 +0.5 +0.3
carbon source succinate (C) N.D. +0.1 +0.3 +0.7 +0.3
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.3 +0.6 +0.0 +0.5
stress Chlorite 1 mM N.D. +0.2 +0.2 +1.0 -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.3 +0.3 +0.4 +0.6
nitrogen source L-Aspartic Acid (N) N.D. +0.1 +0.2 +0.7 +0.5
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