Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS31090 and AZOBR_RS31095 are separated by 3 nucleotidesAZOBR_RS31095 and AZOBR_RS31100 are separated by 237 nucleotidesAZOBR_RS31100 and AZOBR_RS31105 are separated by 133 nucleotidesAZOBR_RS31105 and AZOBR_RS31110 are separated by 11 nucleotides AZOBR_RS31090: AZOBR_RS31090 - pseudouridine synthase, at 339,949 to 340,659 _RS31090 AZOBR_RS31095: AZOBR_RS31095 - hypothetical protein, at 340,663 to 341,670 _RS31095 AZOBR_RS31100: AZOBR_RS31100 - endonuclease III, at 341,908 to 342,300 _RS31100 AZOBR_RS31105: AZOBR_RS31105 - 8-amino-7-oxononanoate synthase, at 342,434 to 343,615 _RS31105 AZOBR_RS31110: AZOBR_RS31110 - hypothetical protein, at 343,627 to 344,415 _RS31110
Group Condition AZOBR_RS31090 AZOBR_RS31095 AZOBR_RS31100 AZOBR_RS31105 AZOBR_RS31110
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.3 +0.4 -0.7 -4.3
motility outer cut, LB soft agar motility assay +0.5 -2.2 -0.9 -0.4 +0.1
carbon source D-Gluconic Acid (C) -0.3 -0.7 -0.8 +0.1 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.5 -0.2 -1.8 -0.2
lb LB +0.1 -0.2 -1.3 -0.6 -0.6
stress 2-pentyl-furan 1.780666667 mM -0.8 -0.4 -0.4 -0.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 -0.6 +0.3 -0.2 -1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.7 -0.5 -0.7 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.7 +0.4 -0.3 -0.1 -0.8
stress 3-octanone 6.3476 mM +0.2 -0.2 -0.3 +0.3 -1.5
carbon source L-Leucine (C) -0.2 -0.2 +0.4 -0.4 -1.0
nitrogen source casaminos (N) +0.2 +0.2 +0.2 -0.4 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 +0.1 +0.1 -0.9 -1.1
stress D-Cycloserine 0.125 mg/ml -0.3 +0.1 +0.4 +0.4 -1.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.7 -0.2 +0.4 -0.4 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -0.3 +0.0 -1.4 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.2 +0.1 +0.4 +0.4 -1.5
stress Nickel (II) chloride 0.5 mM +0.2 -0.5 +0.3 +0.5 -0.9
stress 2-pentyl-furan 2.671 mM +0.5 +0.8 +0.6 +0.2 -2.4
nitrogen source L-Glutamic (N) -0.2 +0.3 -0.1 -0.7 +0.7
motility inner cut, LB soft agar motility assay -0.2 -0.5 -0.5 +0.1 +1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.4 +1.0 -0.3 +0.3
motility inner cut, LB soft agar motility assay +0.5 +0.7 +0.3 +0.5 -1.8
nitrogen source L-Threonine (N) +0.1 +0.6 +0.6 +0.3 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.6 -0.4 -0.2 +1.8
nitrogen source L-Isoleucine (N) -0.7 +0.3 +0.8 -0.3 +0.5
stress 3-octanone 6.3476 mM +0.8 +0.1 +0.7 +0.5 -1.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.1 +0.7 -0.2 -0.7 +0.8
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.4 -0.2 +0.7 -0.1 +0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days +0.5 +0.5 +0.7 +0.8 +0.3
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