Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30755 and AZOBR_RS30760 are separated by 61 nucleotidesAZOBR_RS30760 and AZOBR_RS30770 are separated by 1557 nucleotidesAZOBR_RS30770 and AZOBR_RS30775 are separated by 4 nucleotidesAZOBR_RS30775 and AZOBR_RS30780 are separated by 31 nucleotides AZOBR_RS30755: AZOBR_RS30755 - acylphosphatase, at 261,029 to 261,322 _RS30755 AZOBR_RS30760: AZOBR_RS30760 - purine permease, at 261,384 to 262,745 _RS30760 AZOBR_RS30770: AZOBR_RS30770 - carbon-nitrogen hydrolase, at 264,303 to 265,109 _RS30770 AZOBR_RS30775: AZOBR_RS30775 - agmatinase, at 265,114 to 266,169 _RS30775 AZOBR_RS30780: AZOBR_RS30780 - spermidine/putrescine ABC transporter permease, at 266,201 to 266,995 _RS30780
Group Condition AZOBR_RS30755 AZOBR_RS30760 AZOBR_RS30770 AZOBR_RS30775 AZOBR_RS30780
stress 2-pentyl-furan 1.780666667 mM N.D. -0.2 -0.9 -0.0 -0.0
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.2 -0.6 -0.0 -0.3
stress 3-octanone 6.3476 mM N.D. -0.5 -0.9 +0.0 +0.3
stress 3-octanone 5.2896 mM N.D. -0.1 -0.6 -0.1 -0.1
stress 2-pentyl-furan 1.335528 mM N.D. -0.2 -0.5 -0.3 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.1 -0.6 +0.2 -0.1
stress 6-Pentyl-2H-pyran-2-one 0.5570485 mM N.D. -0.2 -0.4 +0.1 -0.2
stress Benzethonium chloride 0.003 mM N.D. -0.0 -0.8 -0.1 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 -0.6 +0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 -0.5 +0.2 +0.1
motility inner cut, LB soft agar motility assay N.D. +0.3 -0.6 +0.1 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 +0.4 -0.0 +0.2
stress D-Cycloserine 0.125 mg/ml N.D. +0.1 -0.3 +0.5 +0.0
stress 2-pentyl-furan 1.335528 mM N.D. -0.2 +0.2 +0.2 +0.3
nitrogen source L-Lysine (N) N.D. +0.2 +0.3 +0.1 +0.3
carbon source D-Gluconic Acid (C) N.D. +0.0 +0.3 +0.3 +0.2
nitrogen source Urea (N) N.D. +0.4 +0.2 +0.2 +0.1
nitrogen source L-Glutamic (N) N.D. +0.1 +0.1 +0.6 -0.0
nitrogen source L-Threonine (N) N.D. +0.1 +0.5 +0.1 +0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.6 +0.0 +0.4 -0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.3 +0.2 +0.5 -0.0
nitrogen source L-Serine (N) N.D. -0.0 +0.5 +0.2 +0.3
carbon source Glycerol (C) N.D. +0.3 +0.4 +0.3 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +0.4 +0.3 +0.5 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +0.9 +0.2 +0.3 -0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +0.4 +0.5 +0.4 +0.1
anaerobic; rich media LB; anaerobic N.D. +0.3 +0.5 +0.5 +0.1
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days N.D. +0.5 +0.8 -0.0 +0.5
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +0.5 +0.5 +0.5 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +0.9 +0.6 +0.7 +0.2
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