Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30625 and AZOBR_RS30630 are separated by 133 nucleotidesAZOBR_RS30630 and AZOBR_RS30635 are separated by 111 nucleotidesAZOBR_RS30635 and AZOBR_RS30640 overlap by 38 nucleotidesAZOBR_RS30640 and AZOBR_RS30645 are separated by 269 nucleotides AZOBR_RS30625: AZOBR_RS30625 - MFS transporter, at 226,798 to 228,033 _RS30625 AZOBR_RS30630: AZOBR_RS30630 - transcriptional regulator, at 228,167 to 229,102 _RS30630 AZOBR_RS30635: AZOBR_RS30635 - phosphohydrolase, at 229,214 to 229,675 _RS30635 AZOBR_RS30640: AZOBR_RS30640 - hypothetical protein, at 229,638 to 230,405 _RS30640 AZOBR_RS30645: AZOBR_RS30645 - GTP-binding protein YchF, at 230,675 to 231,775 _RS30645
Group Condition AZOBR_RS30625 AZOBR_RS30630 AZOBR_RS30635 AZOBR_RS30640 AZOBR_RS30645
stress 2-pentyl-furan 2.671 mM -0.2 N.D. -1.1 -0.1 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.2 N.D. -0.1 -0.6 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days -0.2 N.D. -0.2 -0.6 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.1 N.D. -0.1 -0.7 N.D.
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.2 N.D. -0.1 -0.6 N.D.
stress 3-octanone 6.3476 mM -0.1 N.D. -0.1 -0.5 N.D.
motility outer cut, LB soft agar motility assay -0.1 N.D. +0.0 -0.7 N.D.
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.0 N.D. -0.0 -0.7 N.D.
motility inner cut, LB soft agar motility assay +0.1 N.D. -0.4 -0.3 N.D.
stress 2-pentyl-furan 1.780666667 mM +0.3 N.D. -0.5 -0.4 N.D.
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days -0.1 N.D. +0.2 -0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 N.D. -0.5 -0.4 N.D.
anaerobic; rich media LB; anaerobic -0.1 N.D. +0.2 -0.5 N.D.
stress nitrate 100 mM +0.1 N.D. -0.6 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 N.D. -0.3 +0.6 N.D.
nitrogen source L-Isoleucine (N) -0.3 N.D. +0.0 +0.4 N.D.
carbon source L-Proline (C) -0.3 N.D. +0.4 +0.1 N.D.
nitrogen source L-Threonine (N) +0.3 N.D. -0.3 +0.2 N.D.
stress Cobalt chloride 0.2 mM +0.1 N.D. +0.5 -0.3 N.D.
stress 2-pentyl-furan 1.780666667 mM +0.2 N.D. +0.6 -0.4 N.D.
carbon source Glycerol (C) +0.0 N.D. -0.2 +0.6 N.D.
nitrogen source Parabanic Acid (N) +0.2 N.D. -0.2 +0.4 N.D.
stress D-Cycloserine 0.125 mg/ml +0.1 N.D. +0.7 -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 N.D. +0.9 -0.0 N.D.
carbon source Glycerol (C) +0.1 N.D. +0.0 +0.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 N.D. +0.7 -0.1 N.D.
carbon source D-Gluconic Acid (C) +0.4 N.D. +0.2 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 N.D. +0.6 +0.0 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.3 N.D. +0.7 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 N.D. -0.0 +0.9 N.D.
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