Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30595 and AZOBR_RS30600 are separated by 176 nucleotidesAZOBR_RS30600 and AZOBR_RS30605 are separated by 491 nucleotidesAZOBR_RS30605 and AZOBR_RS30610 are separated by 112 nucleotidesAZOBR_RS30610 and AZOBR_RS30615 are separated by 98 nucleotides AZOBR_RS30595: AZOBR_RS30595 - hypothetical protein, at 221,028 to 221,252 _RS30595 AZOBR_RS30600: AZOBR_RS30600 - polyhydroxyalkanoate synthesis repressor, at 221,429 to 222,067 _RS30600 AZOBR_RS30605: AZOBR_RS30605 - poly-beta-hydroxybutyrate polymerase, at 222,559 to 223,797 _RS30605 AZOBR_RS30610: AZOBR_RS30610 - acetyl-CoA acetyltransferase, at 223,910 to 225,085 _RS30610 AZOBR_RS30615: AZOBR_RS30615 - acetoacetyl-CoA reductase, at 225,184 to 225,906 _RS30615
Group Condition AZOBR_RS30595 AZOBR_RS30600 AZOBR_RS30605 AZOBR_RS30610 AZOBR_RS30615
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. -1.2 -1.2 -1.4 -0.6
stress Paraquat dichloride 0.04 mg/ml N.D. -2.5 +0.0 -1.3 -0.5
nitrogen source L-Threonine (N) N.D. -0.6 -0.7 -3.2 +0.3
carbon source a-Ketoglutaric (C) N.D. -3.5 -0.9 +0.4 -0.2
nitrogen fixation control D-Glucose (C) with ammonium chloride; anaerobic; 8 days N.D. -2.0 -1.2 +0.0 -0.8
carbon source L-Proline (C) N.D. -1.7 -0.5 -1.5 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. -1.4 -0.5 -0.9 -0.8
carbon source a-Ketoglutaric (C) N.D. -1.8 -0.9 -0.8 +0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. -1.3 -1.7 +0.2 -0.6
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. -1.1 -0.2 -1.2 -0.7
nitrogen source L-Glutamic (N) N.D. +0.8 +0.2 -3.9 -0.3
nitrogen source L-Isoleucine (N) N.D. -0.4 -0.7 -1.6 -0.5
stress 2-pentyl-furan 1.780666667 mM N.D. +0.3 -0.9 -2.5 -0.0
carbon source D-Fructose (C) N.D. +0.1 -0.8 -2.0 -0.4
carbon source D-Gluconic Acid (C) N.D. -0.3 -0.5 -1.4 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.1 +0.2 -1.9 +0.6
carbon source L-Leucine (C) N.D. +0.4 -1.8 +0.1 -0.7
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. -0.8 -0.8 +0.6 -0.9
stress 3-octanone 6.3476 mM N.D. -0.9 -1.5 +0.4 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.2 +0.6 -1.3 +0.2
carbon source L-Proline (C) N.D. -0.7 -0.9 +1.1 -0.2
stress Chlorite 1 mM N.D. +1.8 -0.2 -2.0 -0.1
stress D-Cycloserine 0.125 mg/ml N.D. +1.8 -0.0 -0.7 -0.5
nitrogen source nitrate (N) N.D. +0.5 -0.7 +1.4 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.3 +0.5 +1.3 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.1 +0.9 +0.6 +0.6
lb LB N.D. +1.2 +0.3 +0.7 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.5 +0.7 +1.2 +0.9
stress Cobalt chloride 0.2 mM N.D. +1.3 -0.3 +1.1 -0.6
motility outer cut, LB soft agar motility assay N.D. +0.4 -1.0 +3.0 -0.5
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