Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30380 and AZOBR_RS30385 are separated by 10 nucleotidesAZOBR_RS30385 and AZOBR_RS30390 are separated by 13 nucleotidesAZOBR_RS30390 and AZOBR_RS30395 are separated by 21 nucleotidesAZOBR_RS30395 and AZOBR_RS30400 are separated by 529 nucleotides AZOBR_RS30380: AZOBR_RS30380 - histidine kinase, at 161,564 to 162,280 _RS30380 AZOBR_RS30385: AZOBR_RS30385 - Fis family transcriptional regulator, at 162,291 to 163,634 _RS30385 AZOBR_RS30390: AZOBR_RS30390 - aspartate aminotransferase, at 163,648 to 165,279 _RS30390 AZOBR_RS30395: AZOBR_RS30395 - transporter, at 165,301 to 166,989 _RS30395 AZOBR_RS30400: AZOBR_RS30400 - ABC transporter substrate-binding protein, at 167,519 to 168,865 _RS30400
Group Condition AZOBR_RS30380 AZOBR_RS30385 AZOBR_RS30390 AZOBR_RS30395 AZOBR_RS30400
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -1.5 -0.1 +0.1 -0.0 -0.1
motility outer cut, LB soft agar motility assay -2.0 +0.2 -0.0 +0.1 +0.2
nitrogen source L-Threonine (N) -0.8 +0.0 -0.2 -0.2 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.0 +0.0 -0.3 -0.2
nitrogen source L-Histidine (N) -0.9 +0.1 -0.0 +0.0 -0.1
carbon source succinate (C) -1.2 -0.0 +0.1 +0.1 +0.1
stress Cobalt chloride 0.2 mM -1.1 -0.3 +0.3 +0.2 +0.1
stress D-Cycloserine 0.125 mg/ml -0.8 +0.0 +0.0 +0.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.7 -0.0 -0.1 +0.3
stress 2-pentyl-furan 1.780666667 mM +0.1 -0.6 -0.1 -0.2 +0.1
nitrogen source casaminos (N) -1.0 +0.2 -0.0 +0.2 +0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.4 -0.3 +0.2 +0.1 +0.0
stress Paraquat dichloride 0.02 mg/ml -0.9 +0.2 +0.1 +0.0 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.8 +0.1 +0.2 -0.0 +0.1
nitrogen source L-Threonine (N) -0.6 +0.6 -0.0 -0.1 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.0 +0.0 -0.3 -0.4
anaerobic; rich media LB; anaerobic -0.5 +0.2 +0.2 +0.0 +0.2
stress Sisomicin 0.01 mg/ml -0.4 +0.3 +0.2 +0.2 -0.0
lb no stress control LB no stress control +0.6 +0.1 -0.0 -0.2 -0.2
stress 3-octanone 5.2896 mM +0.6 -0.1 -0.1 -0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 -0.5 +0.1 +0.3 -0.2
stress 2-pentyl-furan 1.335528 mM +0.4 -0.0 -0.2 +0.2 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.1 +0.4 +0.4 +0.2 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 +0.3 -0.1 -0.0 +0.1
carbon source pyruvate (C) +0.7 +0.2 +0.1 -0.1 +0.1
stress 2-Furfuraldehyde 0.2 vol% +1.0 +0.0 +0.1 -0.1 +0.1
stress Cobalt chloride 0.3 mM +0.7 +0.3 -0.0 +0.0 +0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.4 +0.1 +0.5 +0.3 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 +0.3 +0.1 +0.0 +0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +1.0 +0.3 +0.2 +0.1 +0.2
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