Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30140 and AZOBR_RS30145 are separated by 149 nucleotidesAZOBR_RS30145 and AZOBR_RS30150 are separated by 80 nucleotidesAZOBR_RS30150 and AZOBR_RS30155 are separated by 171 nucleotidesAZOBR_RS30155 and AZOBR_RS30160 are separated by 501 nucleotides AZOBR_RS30140: AZOBR_RS30140 - multidrug transporter, at 101,150 to 102,022 _RS30140 AZOBR_RS30145: AZOBR_RS30145 - formate transporter, at 102,172 to 103,041 _RS30145 AZOBR_RS30150: AZOBR_RS30150 - pyruvate formate lyase-activating protein, at 103,122 to 103,889 _RS30150 AZOBR_RS30155: AZOBR_RS30155 - pyruvate kinase, at 104,061 to 105,494 _RS30155 AZOBR_RS30160: AZOBR_RS30160 - chemotaxis protein CheY, at 105,996 to 108,557 _RS30160
Group Condition AZOBR_RS30140 AZOBR_RS30145 AZOBR_RS30150 AZOBR_RS30155 AZOBR_RS30160
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.0 -0.2 -1.3 -1.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 +0.3 -0.1 -2.7 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.4 +0.5 -2.2 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.1 -0.5 -0.5 -0.6 -0.2
stress Benzethonium chloride 0.003 mM -0.3 +0.1 -0.2 -0.7 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days -0.1 -0.1 -0.5 -0.9 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.4 -0.4 -0.1 -0.6 -0.1
nitrogen source L-Threonine (N) -0.4 -0.4 -0.0 -0.6 -0.2
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.0 -0.3 -0.8 -0.2 -0.1
lb LB -0.2 -0.2 -0.1 -1.0 +0.0
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.1 -0.1 -1.1 -0.2 -0.1
nitrogen source L-Isoleucine (N) +0.1 -0.7 +0.2 -0.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.3 -0.0 -1.8 +0.2
stress 2-pentyl-furan 1.780666667 mM -0.3 -0.9 -0.1 +0.7 +0.1
lb no stress control LB no stress control -0.2 -0.3 -0.3 +0.6 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.2 +0.5 -0.5 +0.2
nitrogen source L-Glutamic (N) +0.3 +0.4 -0.4 +0.2 -0.3
motility inner cut, LB soft agar motility assay +0.1 +0.0 +0.2 +0.7 -0.5
carbon source D-Gluconic Acid (C) +0.3 +0.5 +0.3 -0.9 +0.4
stress 3-octanone 6.3476 mM +0.1 +0.2 -0.2 +1.0 -0.1
stress 2-pentyl-furan 1.780666667 mM +0.2 -0.1 +0.6 +0.6 -0.2
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM -0.1 +0.2 -0.0 +0.9 +0.2
stress Paraquat dichloride 0.02 mg/ml +0.5 -0.2 +0.3 +0.7 -0.2
stress 3-octanone 6.3476 mM -0.3 +0.5 +0.3 +0.6 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 -0.3 +0.3 +1.2 -0.2
stress 2-Furfuraldehyde 0.2 vol% +0.3 +0.4 -0.2 +0.7 +0.2
motility inner cut, LB soft agar motility assay +0.4 +0.7 +0.7 -0.2 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.3 +0.4 +0.4 +0.3
motility outer cut, LB soft agar motility assay +0.4 +0.2 +0.5 +0.7 +0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 +0.5 +0.4 +0.9 -0.2
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