Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30130 and AZOBR_RS30135 are separated by 457 nucleotidesAZOBR_RS30135 and AZOBR_RS30140 are separated by 148 nucleotidesAZOBR_RS30140 and AZOBR_RS30145 are separated by 149 nucleotidesAZOBR_RS30145 and AZOBR_RS30150 are separated by 80 nucleotides AZOBR_RS30130: AZOBR_RS30130 - C4-dicarboxylate ABC transporter, at 98,826 to 99,785 _RS30130 AZOBR_RS30135: AZOBR_RS30135 - GntR family transcriptional regulator, at 100,243 to 101,001 _RS30135 AZOBR_RS30140: AZOBR_RS30140 - multidrug transporter, at 101,150 to 102,022 _RS30140 AZOBR_RS30145: AZOBR_RS30145 - formate transporter, at 102,172 to 103,041 _RS30145 AZOBR_RS30150: AZOBR_RS30150 - pyruvate formate lyase-activating protein, at 103,122 to 103,889 _RS30150
Group Condition AZOBR_RS30130 AZOBR_RS30135 AZOBR_RS30140 AZOBR_RS30145 AZOBR_RS30150
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days -0.0 -0.0 -0.0 -0.2 -1.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.4 -0.1 -0.4 -0.4 -0.1
stress 2-pentyl-furan 1.780666667 mM -0.1 +0.0 -0.3 -0.9 -0.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days +0.1 -0.1 +0.1 -0.1 -1.1
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days +0.2 -0.1 +0.0 -0.3 -0.8
stress 1-octen-3-ol 0.7753276667 mM -0.2 -0.2 -0.2 -0.6 +0.1
nitrogen source L-Isoleucine (N) -0.2 -0.3 +0.1 -0.7 +0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days +0.1 -0.2 -0.2 +0.1 -0.6
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days +0.1 +0.2 +0.1 -0.5 -0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days -0.0 +0.2 -0.1 +0.0 -0.7
stress 2-pentyl-furan 1.780666667 mM +0.2 -0.4 +0.0 -0.5 +0.2
stress 6-Pentyl-2H-pyran-2-one 0.6266795 mM -0.4 -0.2 +0.0 -0.1 +0.3
stress Sisomicin 0.01 mg/ml -0.4 -0.1 +0.2 +0.2 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.2 -0.2 -0.4 +0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.3 +0.3 -0.2 +0.4 -0.1
nitrogen source casaminos (N) -0.1 -0.1 -0.3 +0.5 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.3 +0.2 +0.0 -0.5 +0.2
nitrogen fixation D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days +0.4 +0.1 -0.0 -0.5 +0.2
nitrogen source L-Glutamic (N) +0.3 -0.3 +0.3 +0.4 -0.4
stress Paraquat dichloride 0.02 mg/ml -0.2 -0.0 +0.5 -0.2 +0.3
stress 3-octanone 6.3476 mM -0.1 +0.1 -0.3 +0.5 +0.3
stress 2-pentyl-furan 1.780666667 mM +0.3 -0.2 +0.2 -0.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.0 +0.3 +0.3 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.1 -0.3 +0.2 +0.5
motility outer cut, LB soft agar motility assay -0.0 +0.0 +0.4 +0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.5 +0.3 +0.4 -0.3 +0.3
motility inner cut, LB soft agar motility assay -0.6 +0.2 +0.4 +0.7 +0.7
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.0 +0.2 +0.4 +0.5 +0.4
carbon source D-Gluconic Acid (C) +0.3 +0.1 +0.3 +0.5 +0.3
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.5 +0.5 +0.5 -0.0 +0.4
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