Fitness for 5 genes in Azospirillum brasilense Sp245

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500 ntAZOBR_RS30090 and AZOBR_RS30095 are separated by 129 nucleotidesAZOBR_RS30095 and AZOBR_RS30105 are separated by 1964 nucleotidesAZOBR_RS30105 and AZOBR_RS30120 are separated by 2855 nucleotidesAZOBR_RS30120 and AZOBR_RS30125 overlap by 10 nucleotides AZOBR_RS30090: AZOBR_RS30090 - hypothetical protein, at 90,291 to 90,614 _RS30090 AZOBR_RS30095: AZOBR_RS30095 - hypothetical protein, at 90,744 to 91,787 _RS30095 AZOBR_RS30105: AZOBR_RS30105 - hypothetical protein, at 93,752 to 94,092 _RS30105 AZOBR_RS30120: AZOBR_RS30120 - C4-dicarboxylate ABC transporter permease, at 96,948 to 98,231 _RS30120 AZOBR_RS30125: AZOBR_RS30125 - C4-dicarboxylate ABC transporter permease, at 98,222 to 98,710 _RS30125
Group Condition AZOBR_RS30090 AZOBR_RS30095 AZOBR_RS30105 AZOBR_RS30120 AZOBR_RS30125
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -4.4 N.D. -0.0 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -4.3 N.D. -0.0 +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -4.4 N.D. -0.1 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -2.9 N.D. -0.2 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.5 N.D. -0.1 -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -1.3 N.D. -0.3 -0.9
motility outer cut, LB soft agar motility assay N.D. -0.9 N.D. +0.0 -1.5
nitrogen source Ammonium chloride (N) N.D. -2.4 N.D. +0.0 +0.1
carbon source D-Gluconic Acid (C) N.D. -1.9 N.D. -0.0 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.9 N.D. +0.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.8 N.D. +0.0 -0.2
stress D-Cycloserine 0.125 mg/ml N.D. -1.9 N.D. -0.1 -0.0
nitrogen source casaminos (N) N.D. -1.2 N.D. -0.1 -0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. -0.6 N.D. +0.2 -0.9
stress 2-pentyl-furan 2.671 mM N.D. -1.6 N.D. +0.3 +0.2
nitrogen source L-Histidine (N) N.D. -1.1 N.D. +0.3 -0.3
stress Paraquat dichloride 0.04 mg/ml N.D. -1.3 N.D. +0.1 +0.2
carbon source a-Ketoglutaric (C) N.D. -1.2 N.D. +0.1 +0.2
motility inner cut, LB soft agar motility assay N.D. +0.3 N.D. +0.2 -1.2
nitrogen source L-Isoleucine (N) N.D. -0.6 N.D. -0.3 +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +1.1 N.D. -0.1 -1.0
stress 2-pentyl-furan 1.780666667 mM N.D. +0.7 N.D. -0.5 +0.2
lb LB N.D. +0.8 N.D. -0.1 +0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 3 days N.D. +1.7 N.D. -0.0 -0.5
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days N.D. +1.6 N.D. +0.1 -0.3
motility inner cut, LB soft agar motility assay N.D. +1.3 N.D. +0.2 +0.1
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +2.5 N.D. -0.2 -0.5
nitrogen fixation control L-Malic (C) with ammonium chloride; anaerobic; 8 days N.D. +1.8 N.D. +0.1 -0.0
nitrogen fixation L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 3 days N.D. +2.1 N.D. -0.0 -0.2
nitrogen fixation L-Malic (C) and no Nitrogen; Wolfe's minerals without nitroloacetic acid; 3 days N.D. +2.2 N.D. +0.0 -0.2
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